TY - JOUR
T1 - Conformation and aggregation of a polysaccharide
T2 - In solution, as transported in Golgi vesicles, and in an extracellular matrix
AU - Trachtenberg, S.
PY - 1986
Y1 - 1986
N2 - The structure of the extracellular matrix polysaccharide (ECMP) of Opuntia ficus-indica was studied by transmission and scanning transmission electron microscopy and image analysis. In isolated form, in solution, the ECMP appears long and unbranched. Linear mass measurements indicate that, in solution, aggregation of the polysaccharide chains into bundles occurs. The gelling properties at various pHs and the structure of the gels' junction zones were studied by using Alcian blue 8GX as an artificial gelling agent. The ECMP-Alcian blue junction zones are electrostatic in nature. They are constructed from parallel ECMP strands cross-bridged by Alcian blue molecules with lateral and axial repeats of 30 and 35 Å, respectively. Similar structures can be formed with polygalacturonic, alginic, and hyaluronic acids and heparin. Parallel studies on the polysaccharides morphology, as transported in Golgi vesicles and in the extracellular matrix, were carried out on thin sections stained for polysaccharides.
AB - The structure of the extracellular matrix polysaccharide (ECMP) of Opuntia ficus-indica was studied by transmission and scanning transmission electron microscopy and image analysis. In isolated form, in solution, the ECMP appears long and unbranched. Linear mass measurements indicate that, in solution, aggregation of the polysaccharide chains into bundles occurs. The gelling properties at various pHs and the structure of the gels' junction zones were studied by using Alcian blue 8GX as an artificial gelling agent. The ECMP-Alcian blue junction zones are electrostatic in nature. They are constructed from parallel ECMP strands cross-bridged by Alcian blue molecules with lateral and axial repeats of 30 and 35 Å, respectively. Similar structures can be formed with polygalacturonic, alginic, and hyaluronic acids and heparin. Parallel studies on the polysaccharides morphology, as transported in Golgi vesicles and in the extracellular matrix, were carried out on thin sections stained for polysaccharides.
UR - http://www.scopus.com/inward/record.url?scp=0012745827&partnerID=8YFLogxK
U2 - 10.1016/S0889-1605(86)80009-X
DO - 10.1016/S0889-1605(86)80009-X
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AN - SCOPUS:0012745827
SN - 0889-1605
VL - 97
SP - 89
EP - 102
JO - Journal of Ultrastructure Research and Molecular Structure Research
JF - Journal of Ultrastructure Research and Molecular Structure Research
IS - 1-3
ER -