TY - JOUR
T1 - Conformational switching in the flagellar filament of Salmonella typhimurium
AU - Trachtenberg, Shlomo
AU - DeRosier, David J.
PY - 1992/7/20
Y1 - 1992/7/20
N2 - The flagellar filament of the mutant Salmonella typhimurium strain SJW814 is straight, and has a right-handed twist like the filament of SJW1655. Three-dimensional reconstructions from electron micrographs of ice-embedded filaments reveal a flagellin subunit that has the same domain organization as that of SJW1655. Both show slight changes from the domain organization of the subunits from SJW1660, which possesses a straight, left-handed filament. This points to the possible role of changes in subunit conformation in the left-to-right-handed structural transition in filaments. Comparison of the left and right-handed filaments shows that the subunit's orientation and intersubunit bonding appear to change. The orientation of the subunit in the SJW814 filament is intermediate between that of SJW1655 and SJW1660. Its intermediate orientation may explain why the filaments of SJW1655 and SJW1660 are locked in one conformation, whereas the filament of SJW814 can be induced to switch by, for example, changes in pH and ionic strength.
AB - The flagellar filament of the mutant Salmonella typhimurium strain SJW814 is straight, and has a right-handed twist like the filament of SJW1655. Three-dimensional reconstructions from electron micrographs of ice-embedded filaments reveal a flagellin subunit that has the same domain organization as that of SJW1655. Both show slight changes from the domain organization of the subunits from SJW1660, which possesses a straight, left-handed filament. This points to the possible role of changes in subunit conformation in the left-to-right-handed structural transition in filaments. Comparison of the left and right-handed filaments shows that the subunit's orientation and intersubunit bonding appear to change. The orientation of the subunit in the SJW814 filament is intermediate between that of SJW1655 and SJW1660. Its intermediate orientation may explain why the filaments of SJW1655 and SJW1660 are locked in one conformation, whereas the filament of SJW814 can be induced to switch by, for example, changes in pH and ionic strength.
KW - bacterial flagella
KW - bacterial motility
KW - cryoelectron microscopy
KW - helical three-dimensional reconstruction
KW - switchable protein
UR - http://www.scopus.com/inward/record.url?scp=0026643586&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(92)90959-N
DO - 10.1016/0022-2836(92)90959-N
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C2 - 1640459
AN - SCOPUS:0026643586
SN - 0022-2836
VL - 226
SP - 447
EP - 454
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 2
ER -