TY - JOUR
T1 - Conjugation in tetrahymena pyriformis
T2 - The effect of polylysine, concanavalin a, and bivalent metals on the conjugation process
AU - Ofer, Lea
AU - Levkovitz, Hanna
AU - Loyter, Abraham
PY - 1976/8/1
Y1 - 1976/8/1
N2 - The polycation polylysine, at different degrees of polymerization, was found to cause a marked inhibition of the conjugation process. Inhibition of conjugation by polylysine was highly dependent on the molecular weight of the polymer. When polylysine of a mol wt of 1,250 (degree of polymerization = 6) was used, a concentration of 1.6 × 10-5 M was required for a complete inhibition of conjugation, while only 2 × 10-7 M of polylysine of a mol wt of 71,000 (degree of polymerization = 340) was needed for the same effect. Polyaspartic acid prevented the inhibition of conjugation by polylysine. Chelators of bivalent metals such as O-phenanthroline (10-3 M), EDTA (10-3 M), and EGTA (5 × 10-3 M) strongly inhibit the conjugation process in Tetrahymena pyriformis. The inhibition was partially prevented when bivalent metals such as Zn++ Fe++ and Ca++ were added together with the chelators. The lectin concanavalin A (25 µg/ml) completely prevented the conjugation process, while other lectins, such as phytohemagglutinin (500 µg/ml), soybean agglutinin (75 µg/ml) and wheat germ agglutinin (250 µg/ml) had no effect. Inhibition of conjugation by concanavalin A is completely reversible by 40 mM of α-methyl-D-mannoside.
AB - The polycation polylysine, at different degrees of polymerization, was found to cause a marked inhibition of the conjugation process. Inhibition of conjugation by polylysine was highly dependent on the molecular weight of the polymer. When polylysine of a mol wt of 1,250 (degree of polymerization = 6) was used, a concentration of 1.6 × 10-5 M was required for a complete inhibition of conjugation, while only 2 × 10-7 M of polylysine of a mol wt of 71,000 (degree of polymerization = 340) was needed for the same effect. Polyaspartic acid prevented the inhibition of conjugation by polylysine. Chelators of bivalent metals such as O-phenanthroline (10-3 M), EDTA (10-3 M), and EGTA (5 × 10-3 M) strongly inhibit the conjugation process in Tetrahymena pyriformis. The inhibition was partially prevented when bivalent metals such as Zn++ Fe++ and Ca++ were added together with the chelators. The lectin concanavalin A (25 µg/ml) completely prevented the conjugation process, while other lectins, such as phytohemagglutinin (500 µg/ml), soybean agglutinin (75 µg/ml) and wheat germ agglutinin (250 µg/ml) had no effect. Inhibition of conjugation by concanavalin A is completely reversible by 40 mM of α-methyl-D-mannoside.
UR - http://www.scopus.com/inward/record.url?scp=0016991684&partnerID=8YFLogxK
U2 - 10.1083/jcb.70.2.287
DO - 10.1083/jcb.70.2.287
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C2 - 820698
AN - SCOPUS:0016991684
SN - 0021-9525
VL - 70
SP - 287
EP - 293
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 2
ER -