Structural conservation of cytosolic phosphoenolpyruvate carboxykinase protein and mRNA sequence was found in all species examined from rodents to human. The mitochondrial isoenzyme, in all species tested, represents a distinct protein. Moreover, irrespective of the ratio of cytosolic to mitochondrial isoenzyme, cytosolic phosphoenolpyruvate carboxykinase activity in the human as in the rat is controlled at the level of gene expression and through the same multiple hormonal stimulation. This evolutionary conservation of the cytosolic phosphoenolpyruvate carboxykinase structure and mode of regulation supports the enzymes' physiological importance in mammals.
Bibliographical noteFunding Information:
This study was supportedb y a grant from the Israeli National Academy of Sciences and Humanities, agreemenMt CHA/T. We thank Dr R.W. Hanson for the rat PEPCK genomicp E-5.4 and for the cDNA pPCK-10.
- Gene expression
- Phosphoenolpyruvate carboxykinase