TY - JOUR
T1 - Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution
AU - Mauri, Marta
AU - Kirchner, Marieluise
AU - Aharoni, Reuven
AU - Mattioli, Camilla Ciolli
AU - Van Den Bruck, David
AU - Gutkovitch, Nadya
AU - Modepalli, Vengamanaidu
AU - Selbach, Matthias
AU - Moran, Yehu
AU - Chekulaeva, Marina
N1 - Publisher Copyright:
© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Our current knowledge about the mechanisms of miRNA silencing is restricted to few lineages such as vertebrates, arthropods, nematodes and land plants. miRNA-mediated silencing in bilaterian animals is dependent on the proteins of the GW182 family. Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translation and promoting mRNA decay. Further, similarly to bilaterians, GW182 in Nematostella is recruited to the miRNA repression complex via interaction with Argonaute proteins, and functions downstream to repress mRNA. Thus, our work suggests that this mechanism of miRNA-mediated silencing was already active in the last common ancestor of Cnidaria and Bilateria.
AB - Our current knowledge about the mechanisms of miRNA silencing is restricted to few lineages such as vertebrates, arthropods, nematodes and land plants. miRNA-mediated silencing in bilaterian animals is dependent on the proteins of the GW182 family. Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translation and promoting mRNA decay. Further, similarly to bilaterians, GW182 in Nematostella is recruited to the miRNA repression complex via interaction with Argonaute proteins, and functions downstream to repress mRNA. Thus, our work suggests that this mechanism of miRNA-mediated silencing was already active in the last common ancestor of Cnidaria and Bilateria.
UR - http://www.scopus.com/inward/record.url?scp=85014097753&partnerID=8YFLogxK
U2 - 10.1093/nar/gkw792
DO - 10.1093/nar/gkw792
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C2 - 27604873
AN - SCOPUS:85014097753
SN - 0305-1048
VL - 45
SP - 938
EP - 950
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 2
ER -
