Construction of mini-Tn5cyaA′ and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica

Xuanlin Tu, Israel Nisan, Jeff F. Miller, Emanuel Hanski, Ilan Rosenshine*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A mini-Tn5 transposon derivative, mini-Tn5cyaA′, has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of Bordetella pertussis adenylate cyclase toxin (CyaA′). We used this system to mutagenize B. bronchiseptica and we developed a screen for identification of mutants containing cyaA′ translational fusions. This system was used to identify B. bronchiseptica genes that encode surface-exposed and secreted proteins.

Original languageAmerican English
Pages (from-to)119-123
Number of pages5
JournalFEMS Microbiology Letters
Volume205
Issue number1
DOIs
StatePublished - 27 Nov 2001

Bibliographical note

Funding Information:
This work was supported by the European Union Fifth Framework Quality of Life Program (Grant 30600, I.R.), the Israel Science Foundation (Grant 223/99, I.R.), the Max Deutsch Foundation for Applied Research (E.H. and I.R.), NIH (Grant AI38417, J.F.M.) and USDA (Grant 1999-02298, J.F.M.).

Keywords

  • Bordetella bronchiseptica
  • CyaA
  • Mini-Tn5
  • Protein secretion
  • Reporter gene
  • Transposon mutagenesis

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