Construction of mini-Tn5cyaA′ and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica

Xuanlin Tu; Israel Nisan; Jeff F. Miller; Emanuel Hanski; Ilan Rosenshine

doi:10.1016/S0378-1097(01)00451-7

Construction of mini-Tn5cyaA′ and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica

Xuanlin Tu, Israel Nisan, Jeff F. Miller, Emanuel Hanski, Ilan Rosenshine^*

^*Corresponding author for this work

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A mini-Tn5 transposon derivative, mini-Tn5cyaA′, has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of Bordetella pertussis adenylate cyclase toxin (CyaA′). We used this system to mutagenize B. bronchiseptica and we developed a screen for identification of mutants containing cyaA′ translational fusions. This system was used to identify B. bronchiseptica genes that encode surface-exposed and secreted proteins.

Original language	American English
Pages (from-to)	119-123
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	205
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(01)00451-7
State	Published - 27 Nov 2001

Bibliographical note

Funding Information:
This work was supported by the European Union Fifth Framework Quality of Life Program (Grant 30600, I.R.), the Israel Science Foundation (Grant 223/99, I.R.), the Max Deutsch Foundation for Applied Research (E.H. and I.R.), NIH (Grant AI38417, J.F.M.) and USDA (Grant 1999-02298, J.F.M.).

Keywords

Bordetella bronchiseptica
CyaA
Mini-Tn5
Protein secretion
Reporter gene
Transposon mutagenesis

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10.1016/S0378-1097(01)00451-7

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title = "Construction of mini-Tn5cyaA′ and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica",

abstract = "A mini-Tn5 transposon derivative, mini-Tn5cyaA′, has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of Bordetella pertussis adenylate cyclase toxin (CyaA′). We used this system to mutagenize B. bronchiseptica and we developed a screen for identification of mutants containing cyaA′ translational fusions. This system was used to identify B. bronchiseptica genes that encode surface-exposed and secreted proteins.",

keywords = "Bordetella bronchiseptica, CyaA, Mini-Tn5, Protein secretion, Reporter gene, Transposon mutagenesis",

author = "Xuanlin Tu and Israel Nisan and Miller, {Jeff F.} and Emanuel Hanski and Ilan Rosenshine",

note = "Funding Information: This work was supported by the European Union Fifth Framework Quality of Life Program (Grant 30600, I.R.), the Israel Science Foundation (Grant 223/99, I.R.), the Max Deutsch Foundation for Applied Research (E.H. and I.R.), NIH (Grant AI38417, J.F.M.) and USDA (Grant 1999-02298, J.F.M.). ",

year = "2001",

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doi = "10.1016/S0378-1097(01)00451-7",

language = "American English",

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pages = "119--123",

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T1 - Construction of mini-Tn5cyaA′ and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica

AU - Tu, Xuanlin

AU - Nisan, Israel

AU - Miller, Jeff F.

AU - Hanski, Emanuel

AU - Rosenshine, Ilan

N1 - Funding Information: This work was supported by the European Union Fifth Framework Quality of Life Program (Grant 30600, I.R.), the Israel Science Foundation (Grant 223/99, I.R.), the Max Deutsch Foundation for Applied Research (E.H. and I.R.), NIH (Grant AI38417, J.F.M.) and USDA (Grant 1999-02298, J.F.M.).

PY - 2001/11/27

Y1 - 2001/11/27

N2 - A mini-Tn5 transposon derivative, mini-Tn5cyaA′, has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of Bordetella pertussis adenylate cyclase toxin (CyaA′). We used this system to mutagenize B. bronchiseptica and we developed a screen for identification of mutants containing cyaA′ translational fusions. This system was used to identify B. bronchiseptica genes that encode surface-exposed and secreted proteins.

AB - A mini-Tn5 transposon derivative, mini-Tn5cyaA′, has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of Bordetella pertussis adenylate cyclase toxin (CyaA′). We used this system to mutagenize B. bronchiseptica and we developed a screen for identification of mutants containing cyaA′ translational fusions. This system was used to identify B. bronchiseptica genes that encode surface-exposed and secreted proteins.

KW - Bordetella bronchiseptica

KW - CyaA

KW - Mini-Tn5

KW - Protein secretion

KW - Reporter gene

KW - Transposon mutagenesis

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DO - 10.1016/S0378-1097(01)00451-7

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AN - SCOPUS:0035960699

SN - 0378-1097

VL - 205

SP - 119

EP - 123

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Construction of mini-Tn5cyaA′ and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica

Abstract

Bibliographical note

Keywords

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