Construction of synthetic immunogen: Use of new T-helper epitope on malaria circumsporozoite protein

Michael F. Good*, W. Lee Maloy, Milford N. Lunde, Hanah Margalit, James L. Cornetre, Geoffrey L. Smith, Bernard Moss, Louis H. Miller, Jay A. Berzofsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

242 Scopus citations

Abstract

The circumsporozoite (CS) protein of Plasmodium falciparum is the focus of intense efforts to develop an antisporozoite malaria vaccine. Localization of sites for T-cell recognition on this molecule is critical for vaccine design. By using an algorithm designed to predict T-cell sites and a large panel of H-2 congenic mice, a major nonrepetitive T-cell site was located. When a synthetic peptide corresponding to this site was covalently linked to the major B-cell site on the molecule, an immunogen capable of eliciting a high-titer antibody response was formed. This peptide sequence could prime helper T cells for a secondary response to the intact CS protein. The new helper T-cell site is located outside the repetitive region of the CS protein and appears to be the immunodominant T site on the molecule. This approach should be useful in the rational design and construction of vaccines.

Original languageAmerican English
Pages (from-to)1059-1062
Number of pages4
JournalScience
Volume235
Issue number4792
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Dive into the research topics of 'Construction of synthetic immunogen: Use of new T-helper epitope on malaria circumsporozoite protein'. Together they form a unique fingerprint.

Cite this