Controlled electrocatalysis by microperoxidase-11 and Au-nanoparticle superstructures on conductive supports

Three-dimensional superstructures, multilayer-arrays consisting of Au-nanoparticles (13 ± 1 nm) and crosslinked by microperoxidase-11 (MP-11), were assembled on transparent ITO conductive glass supports. The structures of the assemblies were confirmed by spectroscopic and electrochemical analyses, which revealed that each Au-particle is associated with ca. 50 MP-11 molecules. The multilayered MP-11/Au-particle systems act as electrocatalysts for the reduction of H₂O₂. The number of MP-11 | Au-particle layers associated with the electrode controls the resulting electrocatalytic currents. The controllable number of MP-11 | Au-particle layers associated with the electrode enables the control of the effectiveness of the electrocatalytic process and tuning of the sensitivity of the H₂O₂-sensing interface.