TY - JOUR
T1 - Coordinated and sequential transcription of the cyprinid herpesvirus-3 annotated genes
AU - Ilouze, Maya
AU - Dishon, Arnon
AU - Kotler, Moshe
PY - 2012/10
Y1 - 2012/10
N2 - Cyprinid herpesvirus-3 (CyHV-3) is the cause of a fatal disease in carp and koi fish. The disease is seasonal and appears when water temperatures range from 18 to 28 °C. CyHV-3 is a member of the Alloherpesviridae, a family in the Herpesvirales order that encompasses mammalian, avian and reptilian viruses. CyHV-3 is a large double-stranded DNA (dsDNA) herpesvirus with a genome of approximately 295. kbp, divergent from other mammalian, avian and reptilian herpesviruses, but bearing several genes similar to cyprinid herpesvirus-1 (CyHV-1), CyHV-2, anguillid herpesvirus-1 (AngHV-1), ictalurid herpesvirus-1 (IcHV-1) and ranid herpes virus-1 (RaHV-1).Here we show that viral DNA synthesis commences 4-8. h post-infection (p.i.), and is completely inhibited by pre-treatment with cytosine β- d-arabinofuranoside (Ara-C). Transcription of CyHV-3 genes initiates after infection as early as 1-2. h p.i., and precedes viral DNA synthesis. All 156 annotated open reading frames (ORFs) of the CyHV-3 genome are transcribed into RNAs, most of which can be classified into immediate early (IE or α), early (E or β) and late (L or γ) classes, similar to all other herpesviruses. Several ORFs belonging to these groups are clustered along the viral genome.
AB - Cyprinid herpesvirus-3 (CyHV-3) is the cause of a fatal disease in carp and koi fish. The disease is seasonal and appears when water temperatures range from 18 to 28 °C. CyHV-3 is a member of the Alloherpesviridae, a family in the Herpesvirales order that encompasses mammalian, avian and reptilian viruses. CyHV-3 is a large double-stranded DNA (dsDNA) herpesvirus with a genome of approximately 295. kbp, divergent from other mammalian, avian and reptilian herpesviruses, but bearing several genes similar to cyprinid herpesvirus-1 (CyHV-1), CyHV-2, anguillid herpesvirus-1 (AngHV-1), ictalurid herpesvirus-1 (IcHV-1) and ranid herpes virus-1 (RaHV-1).Here we show that viral DNA synthesis commences 4-8. h post-infection (p.i.), and is completely inhibited by pre-treatment with cytosine β- d-arabinofuranoside (Ara-C). Transcription of CyHV-3 genes initiates after infection as early as 1-2. h p.i., and precedes viral DNA synthesis. All 156 annotated open reading frames (ORFs) of the CyHV-3 genome are transcribed into RNAs, most of which can be classified into immediate early (IE or α), early (E or β) and late (L or γ) classes, similar to all other herpesviruses. Several ORFs belonging to these groups are clustered along the viral genome.
KW - Alloherpesviridae
KW - Annotated open reading frames
KW - Cyprinid herpesvirus-3
KW - Koi herpesvirus
KW - Permissive temperature
KW - Transcription
UR - http://www.scopus.com/inward/record.url?scp=84867218775&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2012.07.015
DO - 10.1016/j.virusres.2012.07.015
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C2 - 22841491
AN - SCOPUS:84867218775
SN - 0168-1702
VL - 169
SP - 98
EP - 106
JO - Virus Research
JF - Virus Research
IS - 1
ER -