TY - JOUR
T1 - Copper Induced Conformational Changes of Tripeptide Monolayer Based Impedimetric Biosensor
AU - Mervinetsky, Evgeniy
AU - Alshanski, Israel
AU - Hamo, Yonatan
AU - Sandonas, Leonardo Medrano
AU - Dianat, Arezoo
AU - Buchwald, Jörg
AU - Gutierrez, Rafael
AU - Cuniberti, Gianaurelio
AU - Hurevich, Mattan
AU - Yitzchaik, Shlomo
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Copper ions play a major role in biological processes. Abnormal Cu2+ ions concentrations are associated with various diseases, hence, can be used as diagnostic target. Monitoring copper ion is currently performed by non-portable, expensive and complicated to use equipment. We present a label free and a highly sensitive electrochemical ion-detecting biosensor based on a Gly-Gly-His tripeptide layer that chelate with Cu2+ ions. The proposed sensing mechanism is that the chelation results in conformational changes in the peptide that forms a denser insulating layer that prevents RedOx species transfer to the surface. This chelation event was monitored using various electrochemical methods and surface chemistry analysis and supported by theoretical calculations. We propose a highly sensitive ion-detection biosensor that can detect Cu2+ ions in the pM range with high SNR parameter.
AB - Copper ions play a major role in biological processes. Abnormal Cu2+ ions concentrations are associated with various diseases, hence, can be used as diagnostic target. Monitoring copper ion is currently performed by non-portable, expensive and complicated to use equipment. We present a label free and a highly sensitive electrochemical ion-detecting biosensor based on a Gly-Gly-His tripeptide layer that chelate with Cu2+ ions. The proposed sensing mechanism is that the chelation results in conformational changes in the peptide that forms a denser insulating layer that prevents RedOx species transfer to the surface. This chelation event was monitored using various electrochemical methods and surface chemistry analysis and supported by theoretical calculations. We propose a highly sensitive ion-detection biosensor that can detect Cu2+ ions in the pM range with high SNR parameter.
UR - http://www.scopus.com/inward/record.url?scp=85028376304&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-10288-z
DO - 10.1038/s41598-017-10288-z
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C2 - 28842708
AN - SCOPUS:85028376304
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 9498
ER -