TY - JOUR
T1 - Correlated expression of VH framework and VHidiotypic determinants on T helper cells and on functionally undefined T cells binding group A streptococcal carbohydrate
AU - Eichmann, Klaus
AU - Ben‐Neriah, Yinon
AU - Hetzelberger, Doris
AU - Polke, Christine
AU - Givol, David
AU - Lonai, Peter
PY - 1980/2
Y1 - 1980/2
N2 - Antibodies to framework determinants of the VH and Vλ. fragments of MOPC 315 and antisera to the VH idiotype determinants of the A 5 A antibody were used to analyze the antigen receptors of mouse T (and B) cells. This was done by using the antibodies as inhibitors in (a) an assay in which the binding of radiolabeled streptococcal carbohydrate (A‐CHO) antigen by primed and unprimed T and B cells is determined and (b) an assay in which the helper activity of group A streptococcal vaccine‐primed T cells is determined. The results suggest that the major proportion of primed and unprimed T cells binding A‐CHO (70–9076) exhibit VH framework and VH idiotypic determinants. This population appears to include the helper T cells. A minor proportion of T cells (10–3076) express Vλ‐related framework determinants and lack VHframework and VH idiotypic determinants. This population does not include T helper cells. Taken together, the data suggest that a subpopulation of T cells, including the helper cells, uses entire Ig VH regions as part of their antigen receptor system.
AB - Antibodies to framework determinants of the VH and Vλ. fragments of MOPC 315 and antisera to the VH idiotype determinants of the A 5 A antibody were used to analyze the antigen receptors of mouse T (and B) cells. This was done by using the antibodies as inhibitors in (a) an assay in which the binding of radiolabeled streptococcal carbohydrate (A‐CHO) antigen by primed and unprimed T and B cells is determined and (b) an assay in which the helper activity of group A streptococcal vaccine‐primed T cells is determined. The results suggest that the major proportion of primed and unprimed T cells binding A‐CHO (70–9076) exhibit VH framework and VH idiotypic determinants. This population appears to include the helper T cells. A minor proportion of T cells (10–3076) express Vλ‐related framework determinants and lack VHframework and VH idiotypic determinants. This population does not include T helper cells. Taken together, the data suggest that a subpopulation of T cells, including the helper cells, uses entire Ig VH regions as part of their antigen receptor system.
UR - http://www.scopus.com/inward/record.url?scp=0018908169&partnerID=8YFLogxK
U2 - 10.1002/eji.1830100207
DO - 10.1002/eji.1830100207
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C2 - 6154580
AN - SCOPUS:0018908169
SN - 0014-2980
VL - 10
SP - 105
EP - 112
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 2
ER -
