Abstract
Photosystem I (PSI) in barley consists of at least 11 polypeptides of which three have apparent sizes of 15-19 kDa. Two of these polypeptides (subunits III and IV) are constituents of the core complex (CCI), the third is a component of the light-harvesting complex (LHCI). After fractionation of PSI into its CCI and LHCI components, each of the polypeptides has been isolated and its N-terminal region sequenced. We conclude that the gene sequence published for subunit IV of spinach [(1988) FEBS Lett. 237, 108-112] is not that of subunit IV but rather that of the 17 kDA LHCIc pigment protein. We confirm that the published sequence for subunit III [(1988) Curr. Genet. 14, 511-518] is indeed that of subunit III; seemingly conflicting identifications, based on apparent sizes on SDS-PAGE, of which polypeptides are subunits III and IV are probably explained by subunit III's electrophoretic migration rate being dependent on the solvent. Photosynthesis; Photosystem I subunit; Light-harvesting complex; Protein sequence; Pigment protein.
| Original language | English |
|---|---|
| Pages (from-to) | 150-154 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 256 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 9 Oct 1989 |
Bibliographical note
Funding Information:AcknowledgementsR:e search was supported by grants to J.P.T. from the US Department of Agriculture and the National Science Foundation. S.A. was supported by a McKnight Fellowship, and A.V. by a BARD Fellowship and the Harry Kay Foundation. We wish to thank Dr Audree Fowler and MS Janice Bleibaum for advising and carrying out the amino acid sequence work.