Correlation of the differences in conformation between 2′-5′ and 3′-5′ dinucleoside monophosphates with their behaviour on a Sephadex LH-20 column

The separation of 2′-5′ dinucleoside monophosphates from their 3′-5′ isomers on Sephadex LH-20 column is described. In addition, the CD spectra of all the different dinucleoside monophosphates are given. The correlation of the differences in conformation between the 2′-5′ and the 3′-5′ dinucleoside monophosphates with their behaviour on the Sephadex LH-20 column is discussed. It is shown that nucleotides do not behave on the Sephadex LH-20 column according to the rules of gel filtration and it is suggested that the separation of the different dinucleoside monophosphates is dictated by the conformation, which affects the adsorption of the nucleotidic material to the column.