cot-1, a gene required for hyphal elongation in Neurospora crassa, encodes a protein kinase

Oded Yarden, Michael Plamann, Daniel J. Ebbole, Charles Yanofsky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

186 Scopus citations

Abstract

Neurospora crassa is a filamentous fungus that grows on semisolid media by forming spreading colonies. Mutations at several loci prevent this spreading growth, cot-1 is a temperature sensitive mutant of N. crassa that exhibits restricted colonial growth. At temperatures above 32°C colonies are compact while at lower temperatures growth is indistinguishable from that of the wild type. Restricted colonial growth is due to a defect in hyphal tip elongation and a concomitant increase in hyphal branching. We have isolated a genomic cosmid clone containing the wild type allele of cot-1 by complementation. Sequence analyses suggested that cot-1 encodes a member of the cAMP-dependent protein kinase family. Strains in which we disrupted cot-1 are viable but display restricted colonial growth. Duplication, by ectopic integration of a promoter-containing fragment which includes the first one-third (209 codons) of the structural gene, unexpectedly resulted in restricted colonial growth. Our results suggest that an active COT1 kinase is required for one or more events essential for hyphal elongation.

Original languageAmerican English
Pages (from-to)2159-2166
Number of pages8
JournalEMBO Journal
Volume11
Issue number6
StatePublished - 1992

Keywords

  • Fungal development
  • Hyphal growth
  • cAMP-dependent protein kinase

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