TY - JOUR
T1 - Coupling High-Performance Thin-Layer Chromatography with Bacterial Genotoxicity Bioreporters
AU - Shakibai, Dror
AU - Riegraf, Carolin
AU - Moscovici, Liat
AU - Reifferscheid, Georg
AU - Buchinger, Sebastian
AU - Belkin, Shimshon
N1 - Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/6/4
Y1 - 2019/6/4
N2 - We present an innovative technological platform for monitoring the direct genotoxicity of individual components in complex environmental samples, based on bioluminescent Escherichia coli genotoxicity bioreporters, sprayed onto the surface of a high-performance thin-layer chromatography (HPTLC) plate. These sensor strains harbor plasmid-borne fusions of selected gene promoters of the E. coli SOS DNA repair system to the Photorhabdus luminescens luxABCDE gene cassette, and mark by increased luminescence the presence of potentially DNA-damaging sample components separated on the plate. We demonstrate an "on plate" quantifiable dose-dependent response to several model genotoxicants (without metabolic activation). We further demonstrate the applicability of the system by identifying as genotoxic specific components of HPTLC-separated influent and effluent samples of wastewater treatment plants, thereby alleviating the need for a comprehensive chemical analysis of the sample.
AB - We present an innovative technological platform for monitoring the direct genotoxicity of individual components in complex environmental samples, based on bioluminescent Escherichia coli genotoxicity bioreporters, sprayed onto the surface of a high-performance thin-layer chromatography (HPTLC) plate. These sensor strains harbor plasmid-borne fusions of selected gene promoters of the E. coli SOS DNA repair system to the Photorhabdus luminescens luxABCDE gene cassette, and mark by increased luminescence the presence of potentially DNA-damaging sample components separated on the plate. We demonstrate an "on plate" quantifiable dose-dependent response to several model genotoxicants (without metabolic activation). We further demonstrate the applicability of the system by identifying as genotoxic specific components of HPTLC-separated influent and effluent samples of wastewater treatment plants, thereby alleviating the need for a comprehensive chemical analysis of the sample.
UR - http://www.scopus.com/inward/record.url?scp=85066777935&partnerID=8YFLogxK
U2 - 10.1021/acs.est.9b00921
DO - 10.1021/acs.est.9b00921
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C2 - 31074978
AN - SCOPUS:85066777935
SN - 0013-936X
VL - 53
SP - 6410
EP - 6419
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 11
ER -