Coupling High-Performance Thin-Layer Chromatography with Bacterial Genotoxicity Bioreporters

Dror Shakibai, Carolin Riegraf, Liat Moscovici, Georg Reifferscheid, Sebastian Buchinger, Shimshon Belkin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We present an innovative technological platform for monitoring the direct genotoxicity of individual components in complex environmental samples, based on bioluminescent Escherichia coli genotoxicity bioreporters, sprayed onto the surface of a high-performance thin-layer chromatography (HPTLC) plate. These sensor strains harbor plasmid-borne fusions of selected gene promoters of the E. coli SOS DNA repair system to the Photorhabdus luminescens luxABCDE gene cassette, and mark by increased luminescence the presence of potentially DNA-damaging sample components separated on the plate. We demonstrate an "on plate" quantifiable dose-dependent response to several model genotoxicants (without metabolic activation). We further demonstrate the applicability of the system by identifying as genotoxic specific components of HPTLC-separated influent and effluent samples of wastewater treatment plants, thereby alleviating the need for a comprehensive chemical analysis of the sample.

Original languageEnglish
Pages (from-to)6410-6419
Number of pages10
JournalEnvironmental Science and Technology
Volume53
Issue number11
DOIs
StatePublished - 4 Jun 2019

Bibliographical note

Publisher Copyright:
© 2019 American Chemical Society.

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