Covalent binding of protamine by glutaraldehyde to bioprosthetic tissue: Characterization and anticalcification effect

G. Golomb, V. Ezra

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Calcification is the principal cause of the clinical failure of bioprosthetic heart valves (BHV). The hypothesis of this work was that an impaired balance between positively and negatively charged amino acids, due to the reaction with Lys and Hyl tissue-collagen residues, expose affinity sites to Ca. We further hypothesized that regardless of the cause(s) of BHV calcification, positive charge modification of the tissue will prevent their propensity to calcify. Modification of BHV tissue was obtained by covalently binding protamine sulfate, a polybasic peptide, via glutaraldehyde. The modification procedure resulted in stable, covalent links of approximately 5.3 % w/w protamine with undiminished anticalcification properties, even after long storage. Significant prevention of calcification was exhibited by the p-bound tissue in comparison to BHV tissue, 66.0 and 106.5 (ug/mg calcium, respectively, after 30 days of subdermal implants in rats. The results support our hypotheses, and orthotopical heart valve replacements are required in order to completely evaluate the treatment efficacy and biocompatibility.

Original languageEnglish
Pages (from-to)31-41
Number of pages11
JournalArtificial Cells, Nanomedicine and Biotechnology
Volume20
Issue number1
DOIs
StatePublished - Jan 1992

Fingerprint

Dive into the research topics of 'Covalent binding of protamine by glutaraldehyde to bioprosthetic tissue: Characterization and anticalcification effect'. Together they form a unique fingerprint.

Cite this