Critical Conditions for Adsorption of Calcium Ions onto Dipolar Lipid Membranes

Oren Lotan; Lea Fink; Asaf Shemesh; Carmen Tamburu; Uri Raviv

doi:10.1021/acs.jpca.6b02708

Critical Conditions for Adsorption of Calcium Ions onto Dipolar Lipid Membranes

Oren Lotan, Lea Fink, Asaf Shemesh, Carmen Tamburu, Uri Raviv^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Dipolar lipid membranes may adsorb multivalent ions. The binding constant depends on the type of lipid and ions. In this paper, we focus on the adsorption of calcium ions onto 1,2-dilauroylphosphatidylcholine (DLPC) membrane. Using small-angle-X-ray scattering we found that at ambient room temperature ca. 0.6 mM CaCl₂ is a critical concentration at which calcium ions adsorbed to 30 mg/mL (ca. 48 mM) DLPC membrane. We then determined the structure of the lamellar phases formed at CaCl₂ concentrations below and above the critical concentration and characterized the effect of temperature and incubation time on the adsorption process. Our findings suggest that calcium adsorption to DLPC membranes requires an initial nucleation phase.

Original language	American English
Pages (from-to)	3390-3396
Number of pages	7
Journal	Journal of Physical Chemistry A
Volume	120
Issue number	19
DOIs	https://doi.org/10.1021/acs.jpca.6b02708
State	Published - 19 May 2016

Bibliographical note

Funding Information:
This project was supported by Israel Science Foundation (1372/13) and by the Focal Technology Area Hybrid Nanomaterials program of the Planning and Budgeting Committee of the Israel Council of Higher Education.

Publisher Copyright:
© 2016 American Chemical Society.

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title = "Critical Conditions for Adsorption of Calcium Ions onto Dipolar Lipid Membranes",

abstract = "Dipolar lipid membranes may adsorb multivalent ions. The binding constant depends on the type of lipid and ions. In this paper, we focus on the adsorption of calcium ions onto 1,2-dilauroylphosphatidylcholine (DLPC) membrane. Using small-angle-X-ray scattering we found that at ambient room temperature ca. 0.6 mM CaCl2 is a critical concentration at which calcium ions adsorbed to 30 mg/mL (ca. 48 mM) DLPC membrane. We then determined the structure of the lamellar phases formed at CaCl2 concentrations below and above the critical concentration and characterized the effect of temperature and incubation time on the adsorption process. Our findings suggest that calcium adsorption to DLPC membranes requires an initial nucleation phase.",

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AU - Fink, Lea

AU - Shemesh, Asaf

AU - Tamburu, Carmen

AU - Raviv, Uri

N1 - Funding Information: This project was supported by Israel Science Foundation (1372/13) and by the Focal Technology Area Hybrid Nanomaterials program of the Planning and Budgeting Committee of the Israel Council of Higher Education. Publisher Copyright: © 2016 American Chemical Society.

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N2 - Dipolar lipid membranes may adsorb multivalent ions. The binding constant depends on the type of lipid and ions. In this paper, we focus on the adsorption of calcium ions onto 1,2-dilauroylphosphatidylcholine (DLPC) membrane. Using small-angle-X-ray scattering we found that at ambient room temperature ca. 0.6 mM CaCl2 is a critical concentration at which calcium ions adsorbed to 30 mg/mL (ca. 48 mM) DLPC membrane. We then determined the structure of the lamellar phases formed at CaCl2 concentrations below and above the critical concentration and characterized the effect of temperature and incubation time on the adsorption process. Our findings suggest that calcium adsorption to DLPC membranes requires an initial nucleation phase.

AB - Dipolar lipid membranes may adsorb multivalent ions. The binding constant depends on the type of lipid and ions. In this paper, we focus on the adsorption of calcium ions onto 1,2-dilauroylphosphatidylcholine (DLPC) membrane. Using small-angle-X-ray scattering we found that at ambient room temperature ca. 0.6 mM CaCl2 is a critical concentration at which calcium ions adsorbed to 30 mg/mL (ca. 48 mM) DLPC membrane. We then determined the structure of the lamellar phases formed at CaCl2 concentrations below and above the critical concentration and characterized the effect of temperature and incubation time on the adsorption process. Our findings suggest that calcium adsorption to DLPC membranes requires an initial nucleation phase.

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Critical Conditions for Adsorption of Calcium Ions onto Dipolar Lipid Membranes

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