TY - JOUR
T1 - Critical Conditions for Adsorption of Calcium Ions onto Dipolar Lipid Membranes
AU - Lotan, Oren
AU - Fink, Lea
AU - Shemesh, Asaf
AU - Tamburu, Carmen
AU - Raviv, Uri
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/5/19
Y1 - 2016/5/19
N2 - Dipolar lipid membranes may adsorb multivalent ions. The binding constant depends on the type of lipid and ions. In this paper, we focus on the adsorption of calcium ions onto 1,2-dilauroylphosphatidylcholine (DLPC) membrane. Using small-angle-X-ray scattering we found that at ambient room temperature ca. 0.6 mM CaCl2 is a critical concentration at which calcium ions adsorbed to 30 mg/mL (ca. 48 mM) DLPC membrane. We then determined the structure of the lamellar phases formed at CaCl2 concentrations below and above the critical concentration and characterized the effect of temperature and incubation time on the adsorption process. Our findings suggest that calcium adsorption to DLPC membranes requires an initial nucleation phase.
AB - Dipolar lipid membranes may adsorb multivalent ions. The binding constant depends on the type of lipid and ions. In this paper, we focus on the adsorption of calcium ions onto 1,2-dilauroylphosphatidylcholine (DLPC) membrane. Using small-angle-X-ray scattering we found that at ambient room temperature ca. 0.6 mM CaCl2 is a critical concentration at which calcium ions adsorbed to 30 mg/mL (ca. 48 mM) DLPC membrane. We then determined the structure of the lamellar phases formed at CaCl2 concentrations below and above the critical concentration and characterized the effect of temperature and incubation time on the adsorption process. Our findings suggest that calcium adsorption to DLPC membranes requires an initial nucleation phase.
UR - http://www.scopus.com/inward/record.url?scp=84971287214&partnerID=8YFLogxK
U2 - 10.1021/acs.jpca.6b02708
DO - 10.1021/acs.jpca.6b02708
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C2 - 27128099
AN - SCOPUS:84971287214
SN - 1089-5639
VL - 120
SP - 3390
EP - 3396
JO - Journal of Physical Chemistry A
JF - Journal of Physical Chemistry A
IS - 19
ER -