Critical Conditions for Adsorption of Calcium Ions onto Dipolar Lipid Membranes

Oren Lotan, Lea Fink, Asaf Shemesh, Carmen Tamburu, Uri Raviv*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Dipolar lipid membranes may adsorb multivalent ions. The binding constant depends on the type of lipid and ions. In this paper, we focus on the adsorption of calcium ions onto 1,2-dilauroylphosphatidylcholine (DLPC) membrane. Using small-angle-X-ray scattering we found that at ambient room temperature ca. 0.6 mM CaCl2 is a critical concentration at which calcium ions adsorbed to 30 mg/mL (ca. 48 mM) DLPC membrane. We then determined the structure of the lamellar phases formed at CaCl2 concentrations below and above the critical concentration and characterized the effect of temperature and incubation time on the adsorption process. Our findings suggest that calcium adsorption to DLPC membranes requires an initial nucleation phase.

Original languageAmerican English
Pages (from-to)3390-3396
Number of pages7
JournalJournal of Physical Chemistry A
Volume120
Issue number19
DOIs
StatePublished - 19 May 2016

Bibliographical note

Publisher Copyright:
© 2016 American Chemical Society.

Fingerprint

Dive into the research topics of 'Critical Conditions for Adsorption of Calcium Ions onto Dipolar Lipid Membranes'. Together they form a unique fingerprint.

Cite this