Cross-activating c-Met/β1 integrin complex drives metastasis and invasive resistance in cancer

Arman Jahangiri, Alan Nguyen, Ankush Chandra, Maxim K. Sidorov, Garima Yagnik, Jonathan Rick, Sung Won Han, William Chen, Patrick M. Flanigan, Dina Schneidman-Duhovny, Smita Mascharak, Michael De Lay, Brandon Imber, Catherine C. Park, Kunio Matsumoto, Kan Lu, Gabriele Bergers, Andrej Sali, William A. Weiss, Manish K. Aghi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


The molecular underpinnings of invasion, a hallmark of cancer, have been defined in terms of individual mediators but crucial interactions between these mediators remain undefined. In xenograft models and patient specimens, we identified a c-Met/β1 integrin complex that formed during significant invasive oncologic processes: breast cancer metastases and glioblastoma invasive resistance to antiangiogenic VEGF neutralizing antibody, bevacizumab. Inducing c-Met/β1 complex formation through an engineered inducible heterodimerization system promoted features crucial to overcoming stressors during metastases or antiangiogenic therapy: migration in the primary site, survival under hypoxia, and extravasation out of circulation. c-Met/β1 complex formation was up-regulated by hypoxia, while VEGF binding VEGFR2 sequestered c-Met and β1 integrin, preventing their binding. Complex formation promoted ligand-independent receptor activation, with integrin-linked kinase phosphorylating c-Met and crystallography revealing the c-Met/β1 complex to maintain the high-affinity β1 integrin conformation. Site-directed mutagenesis verified the necessity for c-Met/β1 binding of amino acids predicted by crystallography to mediate their extracellular interaction. Far-Western blotting and sequential immunoprecipitation revealed that c-Met displaced α5 integrin from β1 integrin, creating a complex with much greater affinity for fibronectin (FN) than α5β1. Thus, tumor cells adapt to microenvironmental stressors induced by metastases or bevacizumab by coopting receptors, which normally promote both cell migration modes: chemotaxis, movement toward concentrations of environmental chemoattractants, and haptotaxis, movement controlled by the relative strengths of peripheral adhesions. Tumor cells then redirect these receptors away from their conventional binding partners, forming a powerful structural c-Met/β1 complex whose ligand-independent cross-activation and robust affinity for FN drive invasive oncologic processes.

Original languageAmerican English
Pages (from-to)E8685-E8694
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number41
StatePublished - 10 Oct 2017
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2017, National Academy of Sciences. All rights reserved.


  • Angiogenesis
  • Glioblastoma
  • Hypoxia
  • Invasion
  • Resistance


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