Cross-species microarray hybridizations: a developing tool for studying species diversity

Carmiya Bar-Or*, Henryk Czosnek, Hinanit Koltai

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

92 Scopus citations

Abstract

The use of cross-species hybridization (CSH) to DNA microarrays, in which the target RNA and microarray probe are from different species, has increased in the past few years. CSH is used in comparative, evolutionary and ecological studies of closely related species, and for gene-expression profiling of many species that lack a representative microarray platform. However, unlike species-specific hybridization, CSH is still considered a non-standard use of microarrays. Here, we present the recent developments in the field of CSH for cDNA and oligomer microarray platforms. We discuss issues that influence the quality of CSH results, including platform choice, experiment design and data analysis, and suggest strategies that can lead to improvement of CSH studies to investigate species diversity.

Original languageEnglish
Pages (from-to)200-207
Number of pages8
JournalTrends in Genetics
Volume23
Issue number4
DOIs
StatePublished - Apr 2007

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