Cryo-protective effect of an ice-binding protein derived from Antarctic bacteria

Marco Mangiagalli, Maya Bar-Dolev, Pietro Tedesco, Antonino Natalello, Aleksei Kaleda, Stefania Brocca, Donatella de Pascale, Sandra Pucciarelli, Cristina Miceli, Ido Bravslavsky, Marina Lotti*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Cold environments are populated by organisms able to contravene deleterious effects of low temperature by diverse adaptive strategies, including the production of ice binding proteins (IBPs) that inhibit the growth of ice crystals inside and outside cells. We describe the properties of such a protein (EfcIBP) identified in the metagenome of an Antarctic biological consortium composed of the ciliate Euplotes focardii and psychrophilic non-cultured bacteria. Recombinant EfcIBP can resist freezing without any conformational damage and is moderately heat stable, with a midpoint temperature of 66.4 °C. Tested for its effects on ice, EfcIBP shows an unusual combination of properties not reported in other bacterial IBPs. First, it is one of the best-performing IBPs described to date in the inhibition of ice recrystallization, with effective concentrations in the nanomolar range. Moreover, EfcIBP has thermal hysteresis activity (0.53 °C at 50 μm) and it can stop a crystal from growing when held at a constant temperature within the thermal hysteresis gap. EfcIBP protects purified proteins and bacterial cells from freezing damage when exposed to challenging temperatures. EfcIBP also possesses a potential N-terminal signal sequence for protein transport and a DUF3494 domain that is common to secreted IBPs. These features lead us to hypothesize that the protein is either anchored at the outer cell surface or concentrated around cells to provide survival advantage to the whole cell consortium.

Original languageAmerican English
Pages (from-to)163-177
Number of pages15
JournalFEBS Journal
Volume284
Issue number1
DOIs
StatePublished - 1 Jan 2017

Bibliographical note

Publisher Copyright:
© 2016 Federation of European Biochemical Societies

Keywords

  • Euplotes focardii consortium
  • cold adaptation
  • ice binding protein
  • ice recrystallization inhibition
  • thermal hysteresis

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