Abstract
Drosophila melanogaster embryonic Schneider cell line No.2 cultured in standard Schneider's medium revealed aggregated particles of picornavirus (PCV) clusters located almost exclusively in cytoplasmic vesicles. The PCV particle is about 25 nm in diameter and aggregates are found in graduate degrees of crystallinity. The PCV aggregates are visible as small or larger membrane bound clusters (ca. 2.2 μm in diameter). This limiting membrane is sometimes shown to be associated with cytoplasmic membranes such as endoplasmic reticulum. Membranes are also observed sandwiching single rows of PCV particles (viral lamellae). Membrane thickness is about 7 nm and is found in various locations associated with the particles. In general the degree of the crystallinity extends from scattered free particles to well ordered crystalline arrays. In this communication we propose a possible stepwise model in the formation of the PCV crystals.
Original language | English |
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Pages (from-to) | 23-33 |
Number of pages | 11 |
Journal | Micron (1969) |
Volume | 13 |
Issue number | 1 |
DOIs | |
State | Published - 1982 |
Keywords
- crystalline vesicle
- Drosophila
- electron microscopy
- picornaviruses