TY - JOUR
T1 - Crystallization and preliminary crystallographic analysisof Streptomyces griseus aminopeptidase
AU - Almog, Orna
AU - Greenblatt, Harry M.
AU - Spangin, Ania
AU - Ben-Meir, Daniella
AU - Blumberg, Shmaryahu
AU - Shoham, Gil
PY - 1993/3/5
Y1 - 1993/3/5
N2 - Streptomyces griseus excretes a small molecular mass (30 kDa) aminopeptidase that could be used for various biotechnological applications. This enzyme was isolated from an extracellular protease mixture of Streptomyces griseus (Pronase E, Sigma) and single crystals were obtainedby the vapor diffusion method using polyethylene glycol 4000 as the precipitant. The crystals belong to the tetragonal space group P41212 (P43212), with cell dimensions of a = b = 61·82(3) Å and c = 145·88(4) Å. Thesecrystals are mechanically strong, they are stable in the X-ray beam and they diffract to better than 1·8 Å resolution. The cell dimensions and the cell symmetry are consistent with one molecule in the asymmetric unit and the crystals are suitable for a detailed high-resolution crystallographic analysis. A complete nativedata set to 1·9 Å resolution has been collected on a Rigaku R-AXIS-IIC Imaging Plate Detector system and a heavy-atom derivative search is in progress.
AB - Streptomyces griseus excretes a small molecular mass (30 kDa) aminopeptidase that could be used for various biotechnological applications. This enzyme was isolated from an extracellular protease mixture of Streptomyces griseus (Pronase E, Sigma) and single crystals were obtainedby the vapor diffusion method using polyethylene glycol 4000 as the precipitant. The crystals belong to the tetragonal space group P41212 (P43212), with cell dimensions of a = b = 61·82(3) Å and c = 145·88(4) Å. Thesecrystals are mechanically strong, they are stable in the X-ray beam and they diffract to better than 1·8 Å resolution. The cell dimensions and the cell symmetry are consistent with one molecule in the asymmetric unit and the crystals are suitable for a detailed high-resolution crystallographic analysis. A complete nativedata set to 1·9 Å resolution has been collected on a Rigaku R-AXIS-IIC Imaging Plate Detector system and a heavy-atom derivative search is in progress.
KW - Aminopeptidase
KW - Crystallization
KW - Streptomyces griseus
KW - X-ray analysis
UR - http://www.scopus.com/inward/record.url?scp=0027530898&partnerID=8YFLogxK
U2 - 10.1006/jmbi.1993.1146
DO - 10.1006/jmbi.1993.1146
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C2 - 8450545
AN - SCOPUS:0027530898
SN - 0022-2836
VL - 230
SP - 342
EP - 344
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -