TY - JOUR
T1 - Crystallization of a complex between the Fab fragment of a human immunoglobulin M (IgM) rheumatoid factor (RF-AN) and the Fc fragment of human IgG4
AU - Sohi, M. K.
AU - Corper, A. L.
AU - Wan, T.
AU - Steinitz, M.
AU - Jefferis, R.
AU - Beale, D.
AU - He, M.
AU - Feinstein, A.
AU - Sutton, B. J.
AU - Taussig, M. J.
PY - 1996
Y1 - 1996
N2 - Rheumatoid factors (RF) are the characteristic autoantibodies found in patients with rheumatoid arthritis. They recognize epitopes in the Fc region of immunoglobulin G (IgG) and are often of the IgM isotype. In order to analyse the nature of RF-Fc interactions, we have crystallized a complex between the Fab fragment of a human monoclonal IgM rheumatoid factor (RF-AN) and the Fc fragment of human IgG4. The stoichiometry of the complex within the crystals was found to be 2:1 Fab:Fc. The crystals diffracted X-rays to 0.3 nm resolution, and the space group was C2, with cell dimensions a = 16.03 nm, b = 8.19 nm, c = 6.42 nm, β = 98.3°. We have also determined the sequence of the variable region of the RF-AN light chain, not hitherto reported. This belongs to the V(λ)III-a subgroup and is closely related to the germline gene Humlv318, from which it differs in three amino acid residues. This is the first reported crystallized complex between a human autoantibody and its autoantigen.
AB - Rheumatoid factors (RF) are the characteristic autoantibodies found in patients with rheumatoid arthritis. They recognize epitopes in the Fc region of immunoglobulin G (IgG) and are often of the IgM isotype. In order to analyse the nature of RF-Fc interactions, we have crystallized a complex between the Fab fragment of a human monoclonal IgM rheumatoid factor (RF-AN) and the Fc fragment of human IgG4. The stoichiometry of the complex within the crystals was found to be 2:1 Fab:Fc. The crystals diffracted X-rays to 0.3 nm resolution, and the space group was C2, with cell dimensions a = 16.03 nm, b = 8.19 nm, c = 6.42 nm, β = 98.3°. We have also determined the sequence of the variable region of the RF-AN light chain, not hitherto reported. This belongs to the V(λ)III-a subgroup and is closely related to the germline gene Humlv318, from which it differs in three amino acid residues. This is the first reported crystallized complex between a human autoantibody and its autoantigen.
UR - http://www.scopus.com/inward/record.url?scp=9344248427&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2567.1996.d01-692.x
DO - 10.1046/j.1365-2567.1996.d01-692.x
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C2 - 8881769
AN - SCOPUS:9344248427
SN - 0019-2805
VL - 88
SP - 636
EP - 641
JO - Immunology
JF - Immunology
IS - 4
ER -