TY - JOUR
T1 - Crystals of 70S ribosomes from thermophilic bacteria are suitable for X-ray amalysis at low resolution
AU - Berkovitch-Yellin, Z.
AU - Hansen, H. A.S.
AU - Bennett, W. S.
AU - Sharon, R.
AU - von Boehlen, K.
AU - Volkmann, N.
AU - Piefke, J.
AU - Yonath, A.
AU - Wittmann, H. G.
PY - 1991/3/1
Y1 - 1991/3/1
N2 - Single crystals of 70S ribosomes from Thermus thermophilus and from the wild-type and a mutant of Bacillus stearothermophilus have been obtained. Using synchroton X-ray beam, these crystals diffract to 19 Å and 35 Å, respectively, and can be irradiated at temperatures of 110-120 K for days without noticeable decay. Diffraction data sets to 22 Å and to 33 Å with reasonable R scale were collected from the crystals of 70S from T. thermophilus. The cell dimensions (a = b = 524 Å, c = 315 Å), the symmetry (P41212 or P43212) and the density (1.063 g/cm3) of these crystals indicate that there is one 70S ribosome per asymmetric unit.
AB - Single crystals of 70S ribosomes from Thermus thermophilus and from the wild-type and a mutant of Bacillus stearothermophilus have been obtained. Using synchroton X-ray beam, these crystals diffract to 19 Å and 35 Å, respectively, and can be irradiated at temperatures of 110-120 K for days without noticeable decay. Diffraction data sets to 22 Å and to 33 Å with reasonable R scale were collected from the crystals of 70S from T. thermophilus. The cell dimensions (a = b = 524 Å, c = 315 Å), the symmetry (P41212 or P43212) and the density (1.063 g/cm3) of these crystals indicate that there is one 70S ribosome per asymmetric unit.
UR - http://www.scopus.com/inward/record.url?scp=0026117899&partnerID=8YFLogxK
U2 - 10.1016/0022-0248(91)90887-B
DO - 10.1016/0022-0248(91)90887-B
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AN - SCOPUS:0026117899
SN - 0022-0248
VL - 110
SP - 208
EP - 213
JO - Journal of Crystal Growth
JF - Journal of Crystal Growth
IS - 1-2
ER -