TY - JOUR
T1 - Cucumber mosaic virus movement protein affects sugar metabolism and transport in tobacco and melon plants
AU - Shalitin, D.
AU - Wang, Y.
AU - Omid, A.
AU - Gal-On, A.
AU - Wolf, S.
PY - 2002
Y1 - 2002
N2 - In addition to its influence on plasmodesmal function, tobacco mosaic virus movement protein (TMV-MP) causes an alteration in carbon metabolism in source leaves and in resource partitioning among the various plant organs. The present study was aimed at characterizing the influence of cucumber mosaic virus (CMV)-MP on carbohydrate metabolism and transport in both tobacco and melon plants. Transgenic tobacco plants expressing the CMV-MP had reduced levels of soluble sugars and starch in their source leaves and a significantly reduced root-to-shoot ratio in comparison with control plants. A novel virus-vector system was employed to express the CMV-coat protein (CP), the CMV-MP or the TMV-MP in melon plants. This set of experiments indicated that the viral MPs cause a significant elevation in the proportion of sucrose in the phloem sap collected from petioles of source leaves, whereas this sugar was at very low levels or even absent from the sap of control melon plants. The mode by which the CMV-MP exerts its effect on phloem-sap sugar composition is discussed in terms of possible alterations in the mechanism of phloem loading.
AB - In addition to its influence on plasmodesmal function, tobacco mosaic virus movement protein (TMV-MP) causes an alteration in carbon metabolism in source leaves and in resource partitioning among the various plant organs. The present study was aimed at characterizing the influence of cucumber mosaic virus (CMV)-MP on carbohydrate metabolism and transport in both tobacco and melon plants. Transgenic tobacco plants expressing the CMV-MP had reduced levels of soluble sugars and starch in their source leaves and a significantly reduced root-to-shoot ratio in comparison with control plants. A novel virus-vector system was employed to express the CMV-coat protein (CP), the CMV-MP or the TMV-MP in melon plants. This set of experiments indicated that the viral MPs cause a significant elevation in the proportion of sucrose in the phloem sap collected from petioles of source leaves, whereas this sugar was at very low levels or even absent from the sap of control melon plants. The mode by which the CMV-MP exerts its effect on phloem-sap sugar composition is discussed in terms of possible alterations in the mechanism of phloem loading.
KW - Biomass partitioning
KW - Cucumber mosaic virus
KW - Melon (Cucumis melo L.)
KW - Movement protein
KW - Tobacco (Nicotiana tabacum L.)
KW - Transgenic plants
UR - http://www.scopus.com/inward/record.url?scp=0036319860&partnerID=8YFLogxK
U2 - 10.1046/j.1365-3040.2002.00888.x
DO - 10.1046/j.1365-3040.2002.00888.x
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AN - SCOPUS:0036319860
SN - 0140-7791
VL - 25
SP - 989
EP - 997
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
IS - 8
ER -