Cultivation of Besnoitia besnoiti and evaluation of susceptibility of laboratory animals to cultured parasites

Varda Shkap; E. Pipano; C. Greenblatt

doi:10.1016/0304-4017(87)90002-1

Cultivation of Besnoitia besnoiti and evaluation of susceptibility of laboratory animals to cultured parasites

Varda Shkap^*
, E. Pipano
, C. Greenblatt

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Cultivation of Besnoitia besnoiti on five mammalian cell types showed that Vero (green monkey kidney), L_9²⁹ (mouse fibroblasts) and BEK (bovine embryo kidney) cells were highly susceptible and were almost destroyed after 5 days of incubation, while MDBK (Madin-Darby bovine kidney) and BL (Theileria annulata-infected bovine lymphoid) cells were less affected. Intraperitoneal infection with culture-derived endozoites was fatal for gerbils (Meriones tristrami and sand rats (Psammomys obesus). Rabbits showed fever, conjunctivitis and orchitis, but survived the infection. Mice, rats, guinea pigs, hamsters and Microtus guentheri did not show signs of illness, but developed specific antibodies to B. besnoiti. B. besnoiti subcultivated for 2 years in BL cells remained virulent for gerbils.

Original language	English
Pages (from-to)	169-178
Number of pages	10
Journal	Veterinary Parasitology
Volume	23
Issue number	3-4
DOIs	https://doi.org/10.1016/0304-4017(87)90002-1
State	Published - Feb 1987

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title = "Cultivation of Besnoitia besnoiti and evaluation of susceptibility of laboratory animals to cultured parasites",

abstract = "Cultivation of Besnoitia besnoiti on five mammalian cell types showed that Vero (green monkey kidney), L929 (mouse fibroblasts) and BEK (bovine embryo kidney) cells were highly susceptible and were almost destroyed after 5 days of incubation, while MDBK (Madin-Darby bovine kidney) and BL (Theileria annulata-infected bovine lymphoid) cells were less affected. Intraperitoneal infection with culture-derived endozoites was fatal for gerbils (Meriones tristrami and sand rats (Psammomys obesus). Rabbits showed fever, conjunctivitis and orchitis, but survived the infection. Mice, rats, guinea pigs, hamsters and Microtus guentheri did not show signs of illness, but developed specific antibodies to B. besnoiti. B. besnoiti subcultivated for 2 years in BL cells remained virulent for gerbils.",

author = "Varda Shkap and E. Pipano and C. Greenblatt",

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AU - Shkap, Varda

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AU - Greenblatt, C.

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AB - Cultivation of Besnoitia besnoiti on five mammalian cell types showed that Vero (green monkey kidney), L929 (mouse fibroblasts) and BEK (bovine embryo kidney) cells were highly susceptible and were almost destroyed after 5 days of incubation, while MDBK (Madin-Darby bovine kidney) and BL (Theileria annulata-infected bovine lymphoid) cells were less affected. Intraperitoneal infection with culture-derived endozoites was fatal for gerbils (Meriones tristrami and sand rats (Psammomys obesus). Rabbits showed fever, conjunctivitis and orchitis, but survived the infection. Mice, rats, guinea pigs, hamsters and Microtus guentheri did not show signs of illness, but developed specific antibodies to B. besnoiti. B. besnoiti subcultivated for 2 years in BL cells remained virulent for gerbils.

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Cultivation of Besnoitia besnoiti and evaluation of susceptibility of laboratory animals to cultured parasites

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