Cultivation of Bovine Mesenchymal Stem Cells on Plant-Based Scaffolds in a Macrofluidic Single-Use Bioreactor for Cultured Meat

Gilad Gome*, Benyamin Chak, Shadi Tawil, Dafna Shpatz, Jonathan Giron, Ilan Brajzblat, Chen Weizman, Andrey Grishko, Sharon Schlesinger*, Oded Shoseyov*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Reducing production costs, known as scaling, is a significant obstacle in the advancement of cultivated meat. The cultivation process hinges on several key components, e.g., cells, media, scaffolds, and bioreactors. This study demonstrates an innovative approach, departing from traditional stainless steel or glass bioreactors, by integrating food-grade plant-based scaffolds and thermoplastic film bioreactors. While thermoplastic films are commonly used for constructing fluidic systems, conventional welding methods are cost-prohibitive and lack rapid prototyping capabilities, thus inflating research and development expenses. The developed laser welding technique facilitates contamination-free and leakproof sealing of polyethylene films, enabling the efficient fabrication of macrofluidic systems with various designs and dimensions. By incorporating food-grade plant-based scaffolds, such as rice seeded with bovine mesenchymal stem cells, into these bioreactors, this study demonstrates sterile cell proliferation on scaffolds within macrofluidic systems. This approach not only reduces bioreactor prototyping and construction costs but also addresses the need for scalable solutions in both research and industrial settings. Integrating single-use bioreactors with minimal shear forces and incorporating macro carriers such as puffed rice may further enhance biomass production in a scaled-out model. The use of food-grade plant-based scaffolds aligns with sustainable practices in tissue engineering and cultured-meat production, emphasizing its suitability for diverse applications.

Original languageAmerican English
Article number1361
JournalFoods
Volume13
Issue number9
DOIs
StatePublished - May 2024

Bibliographical note

Publisher Copyright:
© 2024 by the authors.

Keywords

  • bioreactors
  • cultured meat
  • fluidics
  • scaffolds
  • tissue engineering

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