Cu2+-Modified Metal–Organic Framework Nanoparticles: A Peroxidase-Mimicking Nanoenzyme

Wei Hai Chen, Margarita Vázquez-González, Anna Kozell, Alessandro Cecconello, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

177 Scopus citations

Abstract

The synthesis and characterization of UiO-type metal–organic framework nanoparticles (NMOFs) composed of Zr4+ ions bridged by 2,2′-bipyridine-5,5′-dicarboxylic acid ligands and the postmodification of the NMOFs with Cu2+ ions are described. The resulting Cu2+-modified NMOFs, Cu2+-NMOFs, exhibit peroxidase-like catalytic activities reflected by the catalyzed oxidation of Amplex-Red to the fluorescent Resorufin by H2O2, the catalyzed oxidation of dopamine to aminochrome by H2O2, and the catalyzed generation of chemiluminescence in the presence of luminol/H2O2. Also, the Cu2+-NMOFs mimic NADH peroxidase functions and catalyze the oxidation of dihydronicotinamide adenine dinucleotide, NADH, to nicotinamide adenine dinucleotide, NAD+, in the presence of H2O2. The Cu2+-NMOFs-catalyzed generation of chemiluminescence in the presence of luminol/H2O2 is used to develop a glucose sensor by monitoring the H2O2 formed by the aerobic oxidation of glucose to gluconic acid in the presence of glucose oxidase. Furthermore, loading the Cu2+-NMOFs with fluorescein and activating the catalyzed generation of chemiluminescence in the presence of luminol/H2O2 yield an efficient chemiluminescence resonance energy transfer (CRET) process to the fluorescein reflected by the activation of the fluorescence of the dye (λ = 520 nm, CRET efficiency 35%).

Original languageEnglish
Article number1703149
JournalSmall
Volume14
Issue number5
DOIs
StatePublished - 1 Feb 2018

Bibliographical note

Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

  • chemiluminescence resonance energy transfer (CRET)
  • dopamine
  • glucose
  • NADH
  • sensors

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