TY - JOUR
T1 - Cu2+-Modified Metal–Organic Framework Nanoparticles
T2 - A Peroxidase-Mimicking Nanoenzyme
AU - Chen, Wei Hai
AU - Vázquez-González, Margarita
AU - Kozell, Anna
AU - Cecconello, Alessandro
AU - Willner, Itamar
N1 - Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/2/1
Y1 - 2018/2/1
N2 - The synthesis and characterization of UiO-type metal–organic framework nanoparticles (NMOFs) composed of Zr4+ ions bridged by 2,2′-bipyridine-5,5′-dicarboxylic acid ligands and the postmodification of the NMOFs with Cu2+ ions are described. The resulting Cu2+-modified NMOFs, Cu2+-NMOFs, exhibit peroxidase-like catalytic activities reflected by the catalyzed oxidation of Amplex-Red to the fluorescent Resorufin by H2O2, the catalyzed oxidation of dopamine to aminochrome by H2O2, and the catalyzed generation of chemiluminescence in the presence of luminol/H2O2. Also, the Cu2+-NMOFs mimic NADH peroxidase functions and catalyze the oxidation of dihydronicotinamide adenine dinucleotide, NADH, to nicotinamide adenine dinucleotide, NAD+, in the presence of H2O2. The Cu2+-NMOFs-catalyzed generation of chemiluminescence in the presence of luminol/H2O2 is used to develop a glucose sensor by monitoring the H2O2 formed by the aerobic oxidation of glucose to gluconic acid in the presence of glucose oxidase. Furthermore, loading the Cu2+-NMOFs with fluorescein and activating the catalyzed generation of chemiluminescence in the presence of luminol/H2O2 yield an efficient chemiluminescence resonance energy transfer (CRET) process to the fluorescein reflected by the activation of the fluorescence of the dye (λ = 520 nm, CRET efficiency 35%).
AB - The synthesis and characterization of UiO-type metal–organic framework nanoparticles (NMOFs) composed of Zr4+ ions bridged by 2,2′-bipyridine-5,5′-dicarboxylic acid ligands and the postmodification of the NMOFs with Cu2+ ions are described. The resulting Cu2+-modified NMOFs, Cu2+-NMOFs, exhibit peroxidase-like catalytic activities reflected by the catalyzed oxidation of Amplex-Red to the fluorescent Resorufin by H2O2, the catalyzed oxidation of dopamine to aminochrome by H2O2, and the catalyzed generation of chemiluminescence in the presence of luminol/H2O2. Also, the Cu2+-NMOFs mimic NADH peroxidase functions and catalyze the oxidation of dihydronicotinamide adenine dinucleotide, NADH, to nicotinamide adenine dinucleotide, NAD+, in the presence of H2O2. The Cu2+-NMOFs-catalyzed generation of chemiluminescence in the presence of luminol/H2O2 is used to develop a glucose sensor by monitoring the H2O2 formed by the aerobic oxidation of glucose to gluconic acid in the presence of glucose oxidase. Furthermore, loading the Cu2+-NMOFs with fluorescein and activating the catalyzed generation of chemiluminescence in the presence of luminol/H2O2 yield an efficient chemiluminescence resonance energy transfer (CRET) process to the fluorescein reflected by the activation of the fluorescence of the dye (λ = 520 nm, CRET efficiency 35%).
KW - chemiluminescence resonance energy transfer (CRET)
KW - dopamine
KW - glucose
KW - NADH
KW - sensors
UR - http://www.scopus.com/inward/record.url?scp=85037631626&partnerID=8YFLogxK
U2 - 10.1002/smll.201703149
DO - 10.1002/smll.201703149
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C2 - 29205812
AN - SCOPUS:85037631626
SN - 1613-6810
VL - 14
JO - Small
JF - Small
IS - 5
M1 - 1703149
ER -