Cyclic AMP mediated induction of ornithine decarboxylase of glioma and neuroblastoma cells

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Abstract

The activity of ornithine decarboxylase (EC 4.1.1.17; L ornithine carboxy lyase) of C6 BU 1 glioma and N115 neuroblastoma cells increases significantly when confluent cultures are treated with compounds that increase cellular cAMP levels. These include norepinephrine or isoproterenol, and prostaglandin E1 or adenosine, which stimulate ornithine decarboxylase activity in C6 BU 1 glioma and N115 neuroblastoma cells, respectively. Ornithine decarboxylase activity is also elevated in confluent C6 BU 1 glioma cells treated with dibutyryl cAMP and theophylline, or after the glioma cells are fed with a serum depleted medium in the presence of catecholamines and inhibitors of cyclic nucleotide phosphodiesterase. The activity of the enzyme increases 500 to 1000 fold, 1 to 6 hr after stationary phase N115 neuroblastoma cells are fed with a serum free medium, supplemented with phosphodiesterase inhibitors, adenosine, or prostaglandin E1. This stimulation is antagonized by carbamoyl choline and is blocked by actinomycin D or cycloheximide. These results suggest that the synthesis of ornithine decarboxylase of C6 BU 1 glioma and N115 neuroblastoma cells is controlled by cAMP.

Original languageEnglish
Pages (from-to)3087-3091
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number8
DOIs
StatePublished - 1975
Externally publishedYes

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