TY - JOUR
T1 - Cyclic AMP mediated induction of ornithine decarboxylase of glioma and neuroblastoma cells
AU - Bachrach, U.
PY - 1975
Y1 - 1975
N2 - The activity of ornithine decarboxylase (EC 4.1.1.17; L ornithine carboxy lyase) of C6 BU 1 glioma and N115 neuroblastoma cells increases significantly when confluent cultures are treated with compounds that increase cellular cAMP levels. These include norepinephrine or isoproterenol, and prostaglandin E1 or adenosine, which stimulate ornithine decarboxylase activity in C6 BU 1 glioma and N115 neuroblastoma cells, respectively. Ornithine decarboxylase activity is also elevated in confluent C6 BU 1 glioma cells treated with dibutyryl cAMP and theophylline, or after the glioma cells are fed with a serum depleted medium in the presence of catecholamines and inhibitors of cyclic nucleotide phosphodiesterase. The activity of the enzyme increases 500 to 1000 fold, 1 to 6 hr after stationary phase N115 neuroblastoma cells are fed with a serum free medium, supplemented with phosphodiesterase inhibitors, adenosine, or prostaglandin E1. This stimulation is antagonized by carbamoyl choline and is blocked by actinomycin D or cycloheximide. These results suggest that the synthesis of ornithine decarboxylase of C6 BU 1 glioma and N115 neuroblastoma cells is controlled by cAMP.
AB - The activity of ornithine decarboxylase (EC 4.1.1.17; L ornithine carboxy lyase) of C6 BU 1 glioma and N115 neuroblastoma cells increases significantly when confluent cultures are treated with compounds that increase cellular cAMP levels. These include norepinephrine or isoproterenol, and prostaglandin E1 or adenosine, which stimulate ornithine decarboxylase activity in C6 BU 1 glioma and N115 neuroblastoma cells, respectively. Ornithine decarboxylase activity is also elevated in confluent C6 BU 1 glioma cells treated with dibutyryl cAMP and theophylline, or after the glioma cells are fed with a serum depleted medium in the presence of catecholamines and inhibitors of cyclic nucleotide phosphodiesterase. The activity of the enzyme increases 500 to 1000 fold, 1 to 6 hr after stationary phase N115 neuroblastoma cells are fed with a serum free medium, supplemented with phosphodiesterase inhibitors, adenosine, or prostaglandin E1. This stimulation is antagonized by carbamoyl choline and is blocked by actinomycin D or cycloheximide. These results suggest that the synthesis of ornithine decarboxylase of C6 BU 1 glioma and N115 neuroblastoma cells is controlled by cAMP.
UR - http://www.scopus.com/inward/record.url?scp=0016803206&partnerID=8YFLogxK
U2 - 10.1073/pnas.72.8.3087
DO - 10.1073/pnas.72.8.3087
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 171652
AN - SCOPUS:0016803206
SN - 0027-8424
VL - 72
SP - 3087
EP - 3091
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
ER -