Cyclopentane modified FIT-PNA (cpFIT-PNA) probes are reported as highly emissive RNA sensors with the highest reported brightness for FIT-PNAs. Compared to FIT-PNAs, cpFIT-PNAs have improved mismatch discrimination for several pyrimidine-pyrimidine single nucleotide variants (SNVs).
Bibliographical noteFunding Information:
This research was supported by THE ISRAEL SCIENCE FOUNDATION (grant no. 476/17). EY acknowledges the David R. Bloom Center for Pharmacy and the Alex Grass Center for Drug Design and Synthesis of Novel Therapeutics for financial support. The authors are grateful to Prof. Gonen Ashkenasy for help with Tm measurements.
© 2021 The Royal Society of Chemistry.