TY - JOUR
T1 - Cytokine levels in gingival crevicular fluid of erupting primary teeth correlated with systemic disturbances accompanying teething
AU - Shapira, Joseph
AU - Berenstein-Ajzman, Gisela
AU - Engelhard, Dan
AU - Cahan, Sorel
AU - Kalickman, Ina
AU - Barak, Vivian
PY - 2003
Y1 - 2003
N2 - Purpose: The aim of this study was to investigate whether there are increased levels of the inflammatory cytokines IL-1ß, IL-8, and TNFα in the gingival crevicular fluid (GCF) of erupting primary teeth. This increase could explain such clinical manifestations as fever, diarrhea, increased crying, and sleeping and eating disturbances that occur at this time. Methods: Sixteen healthy children aged 5 to 14 months (mean=9.8 months) were examined twice a week over 5 months. Gingival crevicular fluid samples were taken from erupting teeth. As a control, GCF was collected from the same teeth 1 month later. Cytokine production was measured by ELISA. Signs and clinical symptoms were listed. Pearson correlation coefficients were used in the comparisons described below. A paired t test was used to analyze the same variable at different times. Results: Fifty teeth of the 16 children were studied. GCF samples were collected from 21 of these teeth. Statistically significant differences (P<.05) were found with regard to the occurrence of fever, behavioral problems, and coughing during the teething period and the control period. During the control period, 72% of the children did not exhibit any clinical manifestations, whereas during the teething period only 22% of the children did not exhibit any clinical manifestations. The study revealed high levels of inflammatory cytokines during the teething period, with a statistically significant difference in TNFα levels (P<.05) between the teething period and the control period. Correlations were found between cytokine levels and some of the clinical symptoms of teething: IL-1ß and TNFα were correlated with fever and sleep disturbances; IL-1ß and IL-8 were correlated with gastrointestinal disturbances; IL-1ß was correlated with appetite disturbances. Conclusions: Cytonkines appear in the GCF of erupting prmary teeth. The cytokine levels are correlated to some symptoms of teething.
AB - Purpose: The aim of this study was to investigate whether there are increased levels of the inflammatory cytokines IL-1ß, IL-8, and TNFα in the gingival crevicular fluid (GCF) of erupting primary teeth. This increase could explain such clinical manifestations as fever, diarrhea, increased crying, and sleeping and eating disturbances that occur at this time. Methods: Sixteen healthy children aged 5 to 14 months (mean=9.8 months) were examined twice a week over 5 months. Gingival crevicular fluid samples were taken from erupting teeth. As a control, GCF was collected from the same teeth 1 month later. Cytokine production was measured by ELISA. Signs and clinical symptoms were listed. Pearson correlation coefficients were used in the comparisons described below. A paired t test was used to analyze the same variable at different times. Results: Fifty teeth of the 16 children were studied. GCF samples were collected from 21 of these teeth. Statistically significant differences (P<.05) were found with regard to the occurrence of fever, behavioral problems, and coughing during the teething period and the control period. During the control period, 72% of the children did not exhibit any clinical manifestations, whereas during the teething period only 22% of the children did not exhibit any clinical manifestations. The study revealed high levels of inflammatory cytokines during the teething period, with a statistically significant difference in TNFα levels (P<.05) between the teething period and the control period. Correlations were found between cytokine levels and some of the clinical symptoms of teething: IL-1ß and TNFα were correlated with fever and sleep disturbances; IL-1ß and IL-8 were correlated with gastrointestinal disturbances; IL-1ß was correlated with appetite disturbances. Conclusions: Cytonkines appear in the GCF of erupting prmary teeth. The cytokine levels are correlated to some symptoms of teething.
KW - Cytokines
KW - Gingival fluid
KW - Primary tooth eruption
KW - Teething
UR - http://www.scopus.com/inward/record.url?scp=22044458212&partnerID=8YFLogxK
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AN - SCOPUS:22044458212
SN - 0164-1263
VL - 25
SP - 441
EP - 448
JO - Pediatric Dentistry
JF - Pediatric Dentistry
IS - 5
ER -