Cytotoxic activity of an interleukin 2-Pseudomonas exotoxin chimeric protein produced in Escherichia coli

H. Lorberboum-Galski, D. FitzGerald, V. Chaudhary, S. Adhya, I. Pastan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

125 Scopus citations

Abstract

A cDNA clone for human interleukin 2 (IL-2) has been fused to the 5' end of a modified Pseudomonas exotoxin (PE) gene that lacks the sequences encoding the cell recognition domain. The chimeric protein IL-2-PE40 was produced in Escherichia coli. It was extremely toxic to IL-2 receptor-positive cells but had no measurable effect on cells lacking the IL-2 receptor. IL-2-PE40 might be a useful cytotoxic agent in the treatment of diseases involving IL-2 receptor-positive cells and in the treatment of allograft rejection.

Original languageAmerican English
Pages (from-to)1922-1926
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number6
DOIs
StatePublished - 1988
Externally publishedYes

Keywords

  • T cells
  • cancer
  • diabetes
  • leukemia
  • transplantation

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