Cytotoxic activity of an interleukin 2-Pseudomonas exotoxin chimeric protein produced in Escherichia coli

H. Lorberboum-Galski; D. FitzGerald; V. Chaudhary; S. Adhya; I. Pastan

doi:10.1073/pnas.85.6.1922

Cytotoxic activity of an interleukin 2-Pseudomonas exotoxin chimeric protein produced in Escherichia coli

H. Lorberboum-Galski
, D. FitzGerald
, V. Chaudhary
, S. Adhya
, I. Pastan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

127 Scopus citations

Abstract

A cDNA clone for human interleukin 2 (IL-2) has been fused to the 5' end of a modified Pseudomonas exotoxin (PE) gene that lacks the sequences encoding the cell recognition domain. The chimeric protein IL-2-PE₄₀ was produced in Escherichia coli. It was extremely toxic to IL-2 receptor-positive cells but had no measurable effect on cells lacking the IL-2 receptor. IL-2-PE₄₀ might be a useful cytotoxic agent in the treatment of diseases involving IL-2 receptor-positive cells and in the treatment of allograft rejection.

Original language	English
Pages (from-to)	1922-1926
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	85
Issue number	6
DOIs	https://doi.org/10.1073/pnas.85.6.1922
State	Published - 1988
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

T cells
cancer
diabetes
leukemia
transplantation

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10.1073/pnas.85.6.1922

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title = "Cytotoxic activity of an interleukin 2-Pseudomonas exotoxin chimeric protein produced in Escherichia coli",

abstract = "A cDNA clone for human interleukin 2 (IL-2) has been fused to the 5' end of a modified Pseudomonas exotoxin (PE) gene that lacks the sequences encoding the cell recognition domain. The chimeric protein IL-2-PE40 was produced in Escherichia coli. It was extremely toxic to IL-2 receptor-positive cells but had no measurable effect on cells lacking the IL-2 receptor. IL-2-PE40 might be a useful cytotoxic agent in the treatment of diseases involving IL-2 receptor-positive cells and in the treatment of allograft rejection.",

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author = "H. Lorberboum-Galski and D. FitzGerald and V. Chaudhary and S. Adhya and I. Pastan",

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doi = "10.1073/pnas.85.6.1922",

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AU - Lorberboum-Galski, H.

AU - FitzGerald, D.

AU - Chaudhary, V.

AU - Adhya, S.

AU - Pastan, I.

PY - 1988

Y1 - 1988

N2 - A cDNA clone for human interleukin 2 (IL-2) has been fused to the 5' end of a modified Pseudomonas exotoxin (PE) gene that lacks the sequences encoding the cell recognition domain. The chimeric protein IL-2-PE40 was produced in Escherichia coli. It was extremely toxic to IL-2 receptor-positive cells but had no measurable effect on cells lacking the IL-2 receptor. IL-2-PE40 might be a useful cytotoxic agent in the treatment of diseases involving IL-2 receptor-positive cells and in the treatment of allograft rejection.

AB - A cDNA clone for human interleukin 2 (IL-2) has been fused to the 5' end of a modified Pseudomonas exotoxin (PE) gene that lacks the sequences encoding the cell recognition domain. The chimeric protein IL-2-PE40 was produced in Escherichia coli. It was extremely toxic to IL-2 receptor-positive cells but had no measurable effect on cells lacking the IL-2 receptor. IL-2-PE40 might be a useful cytotoxic agent in the treatment of diseases involving IL-2 receptor-positive cells and in the treatment of allograft rejection.

KW - T cells

KW - cancer

KW - diabetes

KW - leukemia

KW - transplantation

UR - https://www.scopus.com/pages/publications/0024121599

U2 - 10.1073/pnas.85.6.1922

DO - 10.1073/pnas.85.6.1922

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AN - SCOPUS:0024121599

SN - 0027-8424

VL - 85

SP - 1922

EP - 1926

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 6

ER -

Cytotoxic activity of an interleukin 2-Pseudomonas exotoxin chimeric protein produced in Escherichia coli

Abstract

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Keywords

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