TY - JOUR
T1 - Cytotoxic effect of normal rabbit serum on human lymphoid cells
AU - Wayne Flye, M.
AU - Schlesinger, Michael
AU - Bernard Amos, D.
PY - 1973/8
Y1 - 1973/8
N2 - Normal rabbit serum contains a complement-dependent natural antibody which is cytotoxic for human lymphoid cells. Thymus cells are most sensitive to its toxicity. The cytotoxic factor is heat-labile at 56 C for 30 min and 2-mercaptoethanol-sensitive. Cytotoxicity was inhibited by goat antirabbit γ-globulin antiserum, heated human serum, preincubation with cobra venom factor, and ethylenediaminetetraacetic acid (EDTA), and removed by absorption with agar or agarose. Serum from other species exhibited varying degrees of cytotoxicity to thymus cells. Treatment of peripheral blood lymphocytes with Vibrio cholerae neuraminidase increased their sensitivity to normal rabbit serum cytotoxicity to nearly that of thymocytes.
AB - Normal rabbit serum contains a complement-dependent natural antibody which is cytotoxic for human lymphoid cells. Thymus cells are most sensitive to its toxicity. The cytotoxic factor is heat-labile at 56 C for 30 min and 2-mercaptoethanol-sensitive. Cytotoxicity was inhibited by goat antirabbit γ-globulin antiserum, heated human serum, preincubation with cobra venom factor, and ethylenediaminetetraacetic acid (EDTA), and removed by absorption with agar or agarose. Serum from other species exhibited varying degrees of cytotoxicity to thymus cells. Treatment of peripheral blood lymphocytes with Vibrio cholerae neuraminidase increased their sensitivity to normal rabbit serum cytotoxicity to nearly that of thymocytes.
UR - http://www.scopus.com/inward/record.url?scp=0015652814&partnerID=8YFLogxK
U2 - 10.1097/00007890-197308000-00006
DO - 10.1097/00007890-197308000-00006
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C2 - 4542410
AN - SCOPUS:0015652814
SN - 0041-1337
VL - 16
SP - 117
EP - 121
JO - Transplantation
JF - Transplantation
IS - 2
ER -