Cytotoxic effect of normal rabbit serum on human lymphoid cells

M. Wayne Flye, Michael Schlesinger, D. Bernard Amos

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Normal rabbit serum contains a complement-dependent natural antibody which is cytotoxic for human lymphoid cells. Thymus cells are most sensitive to its toxicity. The cytotoxic factor is heat-labile at 56 C for 30 min and 2-mercaptoethanol-sensitive. Cytotoxicity was inhibited by goat antirabbit γ-globulin antiserum, heated human serum, preincubation with cobra venom factor, and ethylenediaminetetraacetic acid (EDTA), and removed by absorption with agar or agarose. Serum from other species exhibited varying degrees of cytotoxicity to thymus cells. Treatment of peripheral blood lymphocytes with Vibrio cholerae neuraminidase increased their sensitivity to normal rabbit serum cytotoxicity to nearly that of thymocytes.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalTransplantation
Volume16
Issue number2
DOIs
StatePublished - Aug 1973
Externally publishedYes

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