TY - JOUR
T1 - Cytotoxic effect of oxidized spermine on Ehrlich ascites cells
AU - Bachrach, U.
AU - Abzug, S.
AU - Bekierkunst, A.
PY - 1967/1/25
Y1 - 1967/1/25
N2 - Spermine, after oxidation by highly purified serum amine oxidase (amine: O2 oxidoreductase (deaminating)), inhibited the multiplication of Ehrlich ascites cells. The oxidation product, oxidized spermine, was separated from the amine oxidase by gel filtration and its toxicity verified. The related triamines, spermidine and diaminodipropylamine, were also oxidized by purified serum amine oxidase, giving rise to cytotoxic products. The polyamines in the absence of amine oxidase did not affect the multiplication of the Ehrlich ascites cells, whereas purified amine oxidase alone exhibited a slight cytotoxic effect. Oxidized [14C]spermine was rapidly taken up by Ehrlich ascites cells on incubation at 37°. The incorporation of radioactive valine, thymidine or uracil, into the corresponding cellular macromolecules, was markedly reduced after treating Ehrlich ascites cells with oxidized spermine for 30 min at 37°.
AB - Spermine, after oxidation by highly purified serum amine oxidase (amine: O2 oxidoreductase (deaminating)), inhibited the multiplication of Ehrlich ascites cells. The oxidation product, oxidized spermine, was separated from the amine oxidase by gel filtration and its toxicity verified. The related triamines, spermidine and diaminodipropylamine, were also oxidized by purified serum amine oxidase, giving rise to cytotoxic products. The polyamines in the absence of amine oxidase did not affect the multiplication of the Ehrlich ascites cells, whereas purified amine oxidase alone exhibited a slight cytotoxic effect. Oxidized [14C]spermine was rapidly taken up by Ehrlich ascites cells on incubation at 37°. The incorporation of radioactive valine, thymidine or uracil, into the corresponding cellular macromolecules, was markedly reduced after treating Ehrlich ascites cells with oxidized spermine for 30 min at 37°.
UR - http://www.scopus.com/inward/record.url?scp=0012856360&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(67)90099-8
DO - 10.1016/0005-2787(67)90099-8
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AN - SCOPUS:0012856360
SN - 0005-2787
VL - 134
SP - 174
EP - 181
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 1
ER -