TY - JOUR
T1 - Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris
AU - Burow, Susann
AU - Fontaine, Romain
AU - von Krogh, Kristine
AU - Mayer, Ian
AU - Nourizadeh-Lillabadi, Rasoul
AU - Hollander-Cohen, Lian
AU - Cohen, Yaron
AU - Shpilman, Michal
AU - Levavi-Sivan, Berta
AU - Weltzien, Finn Arne
N1 - Publisher Copyright:
© 2019
PY - 2019/2
Y1 - 2019/2
N2 - The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (Oryzias latipes) (md) gonadotropins Fshβ (mdFshβ), Lhβ (mdLhβ), Fshβα (mdFshβα), and Lhβα (mdLhβα) generated by Pichia pastoris, as well as data on a validation of produced antibodies against Fshβ and Lhβ by Western blot analysis. Furthermore, the article includes data on Fsh and Lh protein levels in male medaka pituitaries using recombinant mdFshβα and mdLhβα within enzyme-linked immunosorbent assays (ELISAs), in which protein amounts were analyzed related to body weight and age of the fish. This dataset is associated with the research article entitled “Medaka Follicle-stimulating hormone (Fsh) and Luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression” (Burow et al., in press).
AB - The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (Oryzias latipes) (md) gonadotropins Fshβ (mdFshβ), Lhβ (mdLhβ), Fshβα (mdFshβα), and Lhβα (mdLhβα) generated by Pichia pastoris, as well as data on a validation of produced antibodies against Fshβ and Lhβ by Western blot analysis. Furthermore, the article includes data on Fsh and Lh protein levels in male medaka pituitaries using recombinant mdFshβα and mdLhβα within enzyme-linked immunosorbent assays (ELISAs), in which protein amounts were analyzed related to body weight and age of the fish. This dataset is associated with the research article entitled “Medaka Follicle-stimulating hormone (Fsh) and Luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression” (Burow et al., in press).
KW - Enzyme-linked immunosorbent assay
KW - Follicle-stimulating hormone
KW - Luteinizing hormone
KW - Oryzias latipes
KW - Recombinant gonadotropins
KW - Western blot analysis
UR - http://www.scopus.com/inward/record.url?scp=85060525778&partnerID=8YFLogxK
U2 - 10.1016/j.dib.2019.01.034
DO - 10.1016/j.dib.2019.01.034
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AN - SCOPUS:85060525778
SN - 2352-3409
VL - 22
SP - 1057
EP - 1063
JO - Data in Brief
JF - Data in Brief
ER -