Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris

Susann Burow; Romain Fontaine; Kristine von Krogh; Ian Mayer; Rasoul Nourizadeh-Lillabadi; Lian Hollander-Cohen; Yaron Cohen; Michal Shpilman; Berta Levavi-Sivan; Finn Arne Weltzien

doi:10.1016/j.dib.2019.01.034

Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris

Susann Burow, Romain Fontaine, Kristine von Krogh, Ian Mayer, Rasoul Nourizadeh-Lillabadi, Lian Hollander-Cohen, Yaron Cohen, Michal Shpilman, Berta Levavi-Sivan, Finn Arne Weltzien^*

^*Corresponding author for this work

Department of Animal Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (Oryzias latipes) (md) gonadotropins Fshβ (mdFshβ), Lhβ (mdLhβ), Fshβα (mdFshβα), and Lhβα (mdLhβα) generated by Pichia pastoris, as well as data on a validation of produced antibodies against Fshβ and Lhβ by Western blot analysis. Furthermore, the article includes data on Fsh and Lh protein levels in male medaka pituitaries using recombinant mdFshβα and mdLhβα within enzyme-linked immunosorbent assays (ELISAs), in which protein amounts were analyzed related to body weight and age of the fish. This dataset is associated with the research article entitled “Medaka Follicle-stimulating hormone (Fsh) and Luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression” (Burow et al., in press).

Original language	American English
Pages (from-to)	1057-1063
Number of pages	7
Journal	Data in Brief
Volume	22
DOIs	https://doi.org/10.1016/j.dib.2019.01.034
State	Published - Feb 2019

Bibliographical note

Funding Information:
This research was supported financially by the Norwegian University of Life Sciences and the Research Council of Norway (Grant number 248828 BioTek2021 , and 231767 FriPro ).

Publisher Copyright:
© 2019

Keywords

Enzyme-linked immunosorbent assay
Follicle-stimulating hormone
Luteinizing hormone
Oryzias latipes
Recombinant gonadotropins
Western blot analysis

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10.1016/j.dib.2019.01.034

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Burow, S., Fontaine, R., von Krogh, K., Mayer, I., Nourizadeh-Lillabadi, R., Hollander-Cohen, L., Cohen, Y., Shpilman, M., Levavi-Sivan, B., & Weltzien, F. A. (2019). Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris. Data in Brief, 22, 1057-1063. https://doi.org/10.1016/j.dib.2019.01.034

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title = "Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris",

abstract = "The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (Oryzias latipes) (md) gonadotropins Fshβ (mdFshβ), Lhβ (mdLhβ), Fshβα (mdFshβα), and Lhβα (mdLhβα) generated by Pichia pastoris, as well as data on a validation of produced antibodies against Fshβ and Lhβ by Western blot analysis. Furthermore, the article includes data on Fsh and Lh protein levels in male medaka pituitaries using recombinant mdFshβα and mdLhβα within enzyme-linked immunosorbent assays (ELISAs), in which protein amounts were analyzed related to body weight and age of the fish. This dataset is associated with the research article entitled “Medaka Follicle-stimulating hormone (Fsh) and Luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression” (Burow et al., in press).",

keywords = "Enzyme-linked immunosorbent assay, Follicle-stimulating hormone, Luteinizing hormone, Oryzias latipes, Recombinant gonadotropins, Western blot analysis",

author = "Susann Burow and Romain Fontaine and {von Krogh}, Kristine and Ian Mayer and Rasoul Nourizadeh-Lillabadi and Lian Hollander-Cohen and Yaron Cohen and Michal Shpilman and Berta Levavi-Sivan and Weltzien, {Finn Arne}",

note = "Funding Information: This research was supported financially by the Norwegian University of Life Sciences and the Research Council of Norway (Grant number 248828 BioTek2021 , and 231767 FriPro ). Publisher Copyright: {\textcopyright} 2019",

year = "2019",

month = feb,

doi = "10.1016/j.dib.2019.01.034",

language = "American English",

volume = "22",

pages = "1057--1063",

journal = "Data in Brief",

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Burow, S, Fontaine, R, von Krogh, K, Mayer, I, Nourizadeh-Lillabadi, R, Hollander-Cohen, L, Cohen, Y, Shpilman, M, Levavi-Sivan, B & Weltzien, FA 2019, 'Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris', Data in Brief, vol. 22, pp. 1057-1063. https://doi.org/10.1016/j.dib.2019.01.034

Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris. / Burow, Susann; Fontaine, Romain; von Krogh, Kristine et al.
In: Data in Brief, Vol. 22, 02.2019, p. 1057-1063.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris

AU - Burow, Susann

AU - Fontaine, Romain

AU - von Krogh, Kristine

AU - Mayer, Ian

AU - Nourizadeh-Lillabadi, Rasoul

AU - Hollander-Cohen, Lian

AU - Cohen, Yaron

AU - Shpilman, Michal

AU - Levavi-Sivan, Berta

AU - Weltzien, Finn Arne

N1 - Funding Information: This research was supported financially by the Norwegian University of Life Sciences and the Research Council of Norway (Grant number 248828 BioTek2021 , and 231767 FriPro ). Publisher Copyright: © 2019

PY - 2019/2

Y1 - 2019/2

N2 - The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (Oryzias latipes) (md) gonadotropins Fshβ (mdFshβ), Lhβ (mdLhβ), Fshβα (mdFshβα), and Lhβα (mdLhβα) generated by Pichia pastoris, as well as data on a validation of produced antibodies against Fshβ and Lhβ by Western blot analysis. Furthermore, the article includes data on Fsh and Lh protein levels in male medaka pituitaries using recombinant mdFshβα and mdLhβα within enzyme-linked immunosorbent assays (ELISAs), in which protein amounts were analyzed related to body weight and age of the fish. This dataset is associated with the research article entitled “Medaka Follicle-stimulating hormone (Fsh) and Luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression” (Burow et al., in press).

AB - The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (Oryzias latipes) (md) gonadotropins Fshβ (mdFshβ), Lhβ (mdLhβ), Fshβα (mdFshβα), and Lhβα (mdLhβα) generated by Pichia pastoris, as well as data on a validation of produced antibodies against Fshβ and Lhβ by Western blot analysis. Furthermore, the article includes data on Fsh and Lh protein levels in male medaka pituitaries using recombinant mdFshβα and mdLhβα within enzyme-linked immunosorbent assays (ELISAs), in which protein amounts were analyzed related to body weight and age of the fish. This dataset is associated with the research article entitled “Medaka Follicle-stimulating hormone (Fsh) and Luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression” (Burow et al., in press).

KW - Enzyme-linked immunosorbent assay

KW - Follicle-stimulating hormone

KW - Luteinizing hormone

KW - Oryzias latipes

KW - Recombinant gonadotropins

KW - Western blot analysis

UR - http://www.scopus.com/inward/record.url?scp=85060525778&partnerID=8YFLogxK

U2 - 10.1016/j.dib.2019.01.034

DO - 10.1016/j.dib.2019.01.034

M3 - Article

AN - SCOPUS:85060525778

SN - 2352-3409

VL - 22

SP - 1057

EP - 1063

JO - Data in Brief

JF - Data in Brief

ER -

Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris

Abstract

Bibliographical note

Keywords

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