DddD is a CoA-transferase/lyase producing dimethyl sulfide in the marine environment

Uria Alcolombri, Paola Laurino, Pedro Lara-Astiaso, Assaf Vardi, Dan S. Tawfik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Dimethyl sulfide (DMS) is produced in oceans in vast amounts (>10 7 tons/year) and mediates a wide range of processes from regulating marine life forms to cloud formation. Nonetheless, none of the enzymes that produce DMS from dimethylsulfoniopropionate (DMSP) has been adequately characterized. We describe the expression and purification of DddD from the marine bacterium Marinomonas sp. MWYL1 and its biochemical characterization. We identified DMSP and acetyl-coenzyme A to be DddD's native substrates and Asp602 as the active site residue mediating the CoA-transferase prior to lyase activity. These findings shed light on the biochemical utilization of DMSP in the marine environment.

Original languageEnglish
Pages (from-to)5473-5475
Number of pages3
JournalBiochemistry
Volume53
Issue number34
DOIs
StatePublished - 2 Sep 2014
Externally publishedYes

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