Deep learning-based virtual refocusing of fluorescence microscopy images for neuron imaging in 3d

Yichen Wu; Yair Rivenson; Hongda Wang; Yilin Luo; Eyal Ben-David; Laurent A. Bentolila; Christian Pritz; Aydogan Ozcan

doi:10.1364/BRAIN.2020.BW4C.6

Deep learning-based virtual refocusing of fluorescence microscopy images for neuron imaging in 3d

Yichen Wu^*
, Yair Rivenson
, Hongda Wang
, Yilin Luo
, Eyal Ben-David
, Laurent A. Bentolila
, Christian Pritz
, Aydogan Ozcan

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We demonstrate a deep neural network-based virtual refocusing method for fluorescence microscopy, which enables accurate tracking of C. elegans neuron structure and activity in 3D using an image acquired at a single focal plane.

Original language	English
Title of host publication	Optics and the Brain, BRAIN 2020
Publisher	Optica Publishing Group (formerly OSA)
ISBN (Print)	9781943580743
DOIs	https://doi.org/10.1364/BRAIN.2020.BW4C.6
State	Published - 2020
Externally published	Yes
Event	Optics and the Brain, BRAIN 2020 - Washington, United States Duration: 20 Apr 2020 → 23 Apr 2020

Publication series

Name	Optics InfoBase Conference Papers
Volume	Part F176-BRAIN-2020
ISSN (Electronic)	2162-2701

Conference

Conference	Optics and the Brain, BRAIN 2020
Country/Territory	United States
City	Washington
Period	20/04/20 → 23/04/20

Bibliographical note

Publisher Copyright:
© OSA 2020 © 2020 The Author(s)

Access to Document

10.1364/BRAIN.2020.BW4C.6

Cite this

Wu, Y., Rivenson, Y., Wang, H., Luo, Y., Ben-David, E., Bentolila, L. A., Pritz, C., & Ozcan, A. (2020). Deep learning-based virtual refocusing of fluorescence microscopy images for neuron imaging in 3d. In Optics and the Brain, BRAIN 2020 (Optics InfoBase Conference Papers; Vol. Part F176-BRAIN-2020). Optica Publishing Group (formerly OSA). https://doi.org/10.1364/BRAIN.2020.BW4C.6

@inproceedings{287fd703f1164ccf97354b2684e67859,

title = "Deep learning-based virtual refocusing of fluorescence microscopy images for neuron imaging in 3d",

abstract = "We demonstrate a deep neural network-based virtual refocusing method for fluorescence microscopy, which enables accurate tracking of C. elegans neuron structure and activity in 3D using an image acquired at a single focal plane.",

author = "Yichen Wu and Yair Rivenson and Hongda Wang and Yilin Luo and Eyal Ben-David and Bentolila, \{Laurent A.\} and Christian Pritz and Aydogan Ozcan",

note = "Publisher Copyright: {\textcopyright} OSA 2020 {\textcopyright} 2020 The Author(s); Optics and the Brain, BRAIN 2020 ; Conference date: 20-04-2020 Through 23-04-2020",

year = "2020",

doi = "10.1364/BRAIN.2020.BW4C.6",

language = "אנגלית",

isbn = "9781943580743",

series = "Optics InfoBase Conference Papers",

publisher = "Optica Publishing Group (formerly OSA)",

booktitle = "Optics and the Brain, BRAIN 2020",

}

Wu, Y, Rivenson, Y, Wang, H, Luo, Y, Ben-David, E, Bentolila, LA, Pritz, C & Ozcan, A 2020, Deep learning-based virtual refocusing of fluorescence microscopy images for neuron imaging in 3d. in Optics and the Brain, BRAIN 2020. Optics InfoBase Conference Papers, vol. Part F176-BRAIN-2020, Optica Publishing Group (formerly OSA), Optics and the Brain, BRAIN 2020, Washington, United States, 20/04/20. https://doi.org/10.1364/BRAIN.2020.BW4C.6

Deep learning-based virtual refocusing of fluorescence microscopy images for neuron imaging in 3d. / Wu, Yichen; Rivenson, Yair; Wang, Hongda et al.
Optics and the Brain, BRAIN 2020. Optica Publishing Group (formerly OSA), 2020. (Optics InfoBase Conference Papers; Vol. Part F176-BRAIN-2020).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Deep learning-based virtual refocusing of fluorescence microscopy images for neuron imaging in 3d

AU - Wu, Yichen

AU - Rivenson, Yair

AU - Wang, Hongda

AU - Luo, Yilin

AU - Ben-David, Eyal

AU - Bentolila, Laurent A.

AU - Pritz, Christian

AU - Ozcan, Aydogan

PY - 2020

Y1 - 2020

N2 - We demonstrate a deep neural network-based virtual refocusing method for fluorescence microscopy, which enables accurate tracking of C. elegans neuron structure and activity in 3D using an image acquired at a single focal plane.

AB - We demonstrate a deep neural network-based virtual refocusing method for fluorescence microscopy, which enables accurate tracking of C. elegans neuron structure and activity in 3D using an image acquired at a single focal plane.

UR - https://www.scopus.com/pages/publications/85091800013

U2 - 10.1364/BRAIN.2020.BW4C.6

DO - 10.1364/BRAIN.2020.BW4C.6

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AN - SCOPUS:85091800013

SN - 9781943580743

T3 - Optics InfoBase Conference Papers

BT - Optics and the Brain, BRAIN 2020

PB - Optica Publishing Group (formerly OSA)

T2 - Optics and the Brain, BRAIN 2020

Y2 - 20 April 2020 through 23 April 2020

ER -

Deep learning-based virtual refocusing of fluorescence microscopy images for neuron imaging in 3d

Abstract

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Conference

Bibliographical note

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