Demonstration and characterization of virus released by murine B cell leukemia cells (BCL1)

Shoshana Morecki; Shimon Slavin; Lola Weiss; Marilyn Weigensberg; Adam Friedman; Moshe Kotler

doi:10.1016/0145-2126(84)90103-6

Demonstration and characterization of virus released by murine B cell leukemia cells (BCL₁)

Shoshana Morecki, Shimon Slavin^*, Lola Weiss, Marilyn Weigensberg, Adam Friedman, Moshe Kotler

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

Abstract

LPS-stimulated leukemia cells derived from BALB/c mice bearing transplantable B-lymphocytic leukemia (BCL₁) which arose spontaneously in a female BALB/c mouse, contained viral particles as suggested by electron microscopy as well as by tissue cultures using the XC assay. Some cell free extracts prepared from BCL₁ cells were capable of transforming normal B-lymphocytes when inoculated into untreated syngeneic recipients. Cytogenetic analysis of spleen cells obtained from a splenomegalic male mouse neonatally inoculated with cell-free extract indicated that the tumor cells had a male karyotype with 42 chromosomes as compared to 37 chromosomes and a female karyotype in original BCL₁ cells, thus excluding the possibility of tumor propagation by transplantation of intact BCL₁ cells. These findings suggest viral involvement in the etiology of BCL₁.

Original language	English
Pages (from-to)	821-825,827-832
Journal	Leukemia Research
Volume	8
Issue number	5
DOIs	https://doi.org/10.1016/0145-2126(84)90103-6
State	Published - 1984

Keywords

B-tropic virus
BCL
leukemogenesis
murine B-cell leukemia
xenotropic virus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0145-2126(84)90103-6

Cite this

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title = "Demonstration and characterization of virus released by murine B cell leukemia cells (BCL1)",

abstract = "LPS-stimulated leukemia cells derived from BALB/c mice bearing transplantable B-lymphocytic leukemia (BCL1) which arose spontaneously in a female BALB/c mouse, contained viral particles as suggested by electron microscopy as well as by tissue cultures using the XC assay. Some cell free extracts prepared from BCL1 cells were capable of transforming normal B-lymphocytes when inoculated into untreated syngeneic recipients. Cytogenetic analysis of spleen cells obtained from a splenomegalic male mouse neonatally inoculated with cell-free extract indicated that the tumor cells had a male karyotype with 42 chromosomes as compared to 37 chromosomes and a female karyotype in original BCL1 cells, thus excluding the possibility of tumor propagation by transplantation of intact BCL1 cells. These findings suggest viral involvement in the etiology of BCL1.",

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author = "Shoshana Morecki and Shimon Slavin and Lola Weiss and Marilyn Weigensberg and Adam Friedman and Moshe Kotler",

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AU - Morecki, Shoshana

AU - Slavin, Shimon

AU - Weiss, Lola

AU - Weigensberg, Marilyn

AU - Friedman, Adam

AU - Kotler, Moshe

PY - 1984

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AB - LPS-stimulated leukemia cells derived from BALB/c mice bearing transplantable B-lymphocytic leukemia (BCL1) which arose spontaneously in a female BALB/c mouse, contained viral particles as suggested by electron microscopy as well as by tissue cultures using the XC assay. Some cell free extracts prepared from BCL1 cells were capable of transforming normal B-lymphocytes when inoculated into untreated syngeneic recipients. Cytogenetic analysis of spleen cells obtained from a splenomegalic male mouse neonatally inoculated with cell-free extract indicated that the tumor cells had a male karyotype with 42 chromosomes as compared to 37 chromosomes and a female karyotype in original BCL1 cells, thus excluding the possibility of tumor propagation by transplantation of intact BCL1 cells. These findings suggest viral involvement in the etiology of BCL1.

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