An 18-mer oligonucleotide almost exclusively targeting Bdellovibrio spp. was designed based on available 16S rRNA sequence data. The specificity of this oligonucleotide used as a PCR primer in combination with a Bacteria domain-targeted primer as well as used as a probe in rRNA dot blot hybridizations was experimentally confirmed using a variety of α-, β-, γ-, δ-Proteobacteria and Gram-positive bacteria. Similarly, combinations of the Bacteria primer with oligonucleotides targeting the 16S rRNA gene of Bdellovibrio bacteriovorus and Bdellovibrio stolpii were shown to be species specific by PCR. Positive amplification products were obtained from an irrigation water sample in which a low level of bdellovibrios was detected by plating as well as from soil detached from potato tubers, using the Bdellovibrio spp.-Bacteria and the B. bacteriovorus-Bacteria primer pairs. Copyright (C) 2000 Federation of European Microbiological Societies.
Bibliographical noteFunding Information:
This work was supported by Chief Scientist grant 823-0138-98 from the Israeli Ministry of Agriculture and by the Israel Science Fundation Grant 132/99.
- 16S rDNA
- Primer design