Designation by restriction fragment length polymorphism of major histocompatibility complex class IV haplotypes in meat‐type chickens

E. Landesman, Z. Uni, E. D. Heller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Major histocompatiblity complex (MHC) class IV haplotypes were identified in a population of meat‐type chickens by restriction fragment length polymorphism (RFLP) analysis. Fourteen different haplotypes were designated on the basis of restriction patterns obtained from Southern blots of PvuII‐ or BglII‐digested DNA, hybridized with the MHC class IV cDNA probe bg32.1. Digestion with each restriction enzyme yielded the same level of polymorphism among individuals. For each haplotype, 4–10 restriction fragments ranging from 0–8 to 8 kb were observed. Such a designation of meat‐type chicken MHC class IV haplotypes enables a rapid recognition of previously defined haplotypes, is readily adjustable to additional, newly found restriction patterns and could prove useful in practical breeding programmes.

Original languageAmerican English
Pages (from-to)349-354
Number of pages6
JournalAnimal Genetics
Volume24
Issue number5
DOIs
StatePublished - Oct 1993

Keywords

  • MHC class IV
  • chicken
  • haplotype
  • restriction fragment length polymorphism

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