Detached leaf enrichment: a method for detecting small numbers of Pseudomonas syringae pv. tomato and Xanthomonas campestris pv. vesicatoria in seed and symptomless leaves of tomato and pepper

Edna Sharon*, Y. Okon, Y. Bashan, Y. Henis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

A method for detecting 101‐102 cells of phytopathogenic bacteria (Pseudomonas syringae pv. tomato and Xanthomonas campestris pv. vesicatoria) in either tomato or pepper seed was developed. The method is based on the enrichment of the compatible pathogen inside a detached leaf of its host when placed on a water agar medium. It was found to be superior to the diagnostic growth media method commonly used and to permit the detection of the pathogens in symptomless plants.

Original languageEnglish
Pages (from-to)371-377
Number of pages7
JournalJournal of Applied Bacteriology
Volume53
Issue number3
DOIs
StatePublished - Dec 1982

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