The Oxford Nanopore (ONT) platform provides portable and rapid genome sequencing, and its ability to natively profile DNA methylation without complex sample processing is attractive for point-of-care real-time sequencing. We recently demonstrated ONT shallow whole-genome sequencing to detect copy number alterations (CNAs) from the circulating tumor DNA (ctDNA) of cancer patients. Here, we show that cell type and cancer-specific methylation changes can also be detected, as well as cancer-associated fragmentation signatures. This feasibility study suggests that ONT shallow WGS could be a powerful tool for liquid biopsy. Graphical Abstract: [Figure not available: see fulltext.]
Bibliographical noteFunding Information:
Ben Berman received startup support from The Hebrew University, the Kamea B program of the Israel Ministry of Aliyah and Immigrant Integration, and the Beethoven Foundation. This work was funded by a project grant to Berman from the Israel Cancer Research Fund Project Grant (Grant #845755). Filippo Martignano was supported by the Italian Ministry of Health (SG-2019-12370279).
© 2022, The Author(s).