Abstract
Two chemical procedures are described for the determination of a myocardial depressant factor (MDF) in the plasma of animals in circulatory shock. These spectrofluorometric and paper chromatographic methods were compared with the standard bioassay of MDF, by cat papillary muscle preparation, with respect to a variety of performance characteristics. The two chemical methods yield values which correlate very closely with the bioassay results, are reproducible and have a sensitivity at least as good as the bioassay method. The chromatographic method is very specific, even more so than the bioassay, whereas the specificity of the spectrofluorometric method is not very high. The other major advantages of the chemical methods are that they require much smaller samples than the bioassay, and that many more plasma samples can be conveniently assayed at one time.
Original language | English |
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Pages (from-to) | 640-647 |
Number of pages | 8 |
Journal | Israel Journal of Medical Sciences |
Volume | 9 |
Issue number | 5 |
State | Published - 1973 |