TY - JOUR
T1 - Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system
AU - Isensee, Kirsten
AU - Erez, Jonathan
AU - Stoll, Heather M.
PY - 2014/2
Y1 - 2014/2
N2 - Accumulation of an intracellular pool of carbon (Ci pool) is one strategy by which marine algae overcome the low abundance of dissolved CO2 (CO2(aq)) in modern seawater. To identify the environmental conditions under which algae accumulate an acid-labile Ci pool, we applied a 14C pulse-chase method, used originally in dinoflagellates, to two new classes of algae, coccolithophorids and diatoms. This method measures the carbon accumulation inside the cells without altering the medium carbon chemistry or culture cell density. We found that the diatom Thalassiosira weissflogii [(Grunow) G. Fryxell & Hasle] and a calcifying strain of the coccolithophorid Emiliania huxleyi [(Lohmann) W. W. Hay & H. P. Mohler] develop significant acid-labile Ci pools. Ci pools are measureable in cells cultured in media with 2-30μmol l-1 CO2(aq), corresponding to a medium pH of 8.6-7.9. The absolute Ci pool was greater for the larger celled diatoms. For both algal classes, the Ci pool became a negligible contributor to photosynthesis once CO2(aq) exceeded 30μmol l-1. Combining the 14C pulse-chase method and 14C disequilibrium method enabled us to assess whether E. huxleyi and T. weissflogii exhibited thresholds for foregoing accumulation of DIC or reduced the reliance on bicarbonate uptake with increasing CO2(aq). We showed that the Ci pool decreases with higher CO2:HCO3- uptake rates.
AB - Accumulation of an intracellular pool of carbon (Ci pool) is one strategy by which marine algae overcome the low abundance of dissolved CO2 (CO2(aq)) in modern seawater. To identify the environmental conditions under which algae accumulate an acid-labile Ci pool, we applied a 14C pulse-chase method, used originally in dinoflagellates, to two new classes of algae, coccolithophorids and diatoms. This method measures the carbon accumulation inside the cells without altering the medium carbon chemistry or culture cell density. We found that the diatom Thalassiosira weissflogii [(Grunow) G. Fryxell & Hasle] and a calcifying strain of the coccolithophorid Emiliania huxleyi [(Lohmann) W. W. Hay & H. P. Mohler] develop significant acid-labile Ci pools. Ci pools are measureable in cells cultured in media with 2-30μmol l-1 CO2(aq), corresponding to a medium pH of 8.6-7.9. The absolute Ci pool was greater for the larger celled diatoms. For both algal classes, the Ci pool became a negligible contributor to photosynthesis once CO2(aq) exceeded 30μmol l-1. Combining the 14C pulse-chase method and 14C disequilibrium method enabled us to assess whether E. huxleyi and T. weissflogii exhibited thresholds for foregoing accumulation of DIC or reduced the reliance on bicarbonate uptake with increasing CO2(aq). We showed that the Ci pool decreases with higher CO2:HCO3- uptake rates.
UR - http://www.scopus.com/inward/record.url?scp=84892488458&partnerID=8YFLogxK
U2 - 10.1111/ppl.12096
DO - 10.1111/ppl.12096
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C2 - 23992373
AN - SCOPUS:84892488458
SN - 0031-9317
VL - 150
SP - 321
EP - 338
JO - Physiologia Plantarum
JF - Physiologia Plantarum
IS - 2
ER -