Detection of aac(6′)-Ib-cr in KPC-producing Klebsiella pneumoniae isolates from Tel Aviv, Israel

Inna Chmelnitsky; Orit Hermesh; Shiri Navon-Venezia; Jacob Strahilevitz; Yehuda Carmeli

doi:10.1093/jac/dkp272

Detection of aac(6′)-Ib-cr in KPC-producing Klebsiella pneumoniae isolates from Tel Aviv, Israel

Inna Chmelnitsky^*
, Orit Hermesh
, Shiri Navon-Venezia
, Jacob Strahilevitz
, Yehuda Carmeli

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Objectives: We aimed to evaluate the occurrence and characteristics of plasmid-mediated quinolone resistance (PMQR) genes in KPC-producing (KPC-P) Klebsiella pneumoniae (Kpn) isolates in Tel Aviv Medical Center, Israel. Methods: Forty-seven KPC-P Kpn isolates were studied. Antibiotic susceptibilities were determined by Vitek 2, Etest or agar dilution. β-Lactamases and PMQR determinants were detected by PCR. For plasmid characterization, transformation, transconjugation, restriction mapping and Southern blot analysis were performed. Results: Six out of 47 (13%) KPC-P isolates carried aac(6′)-Ib-cr. Acquisition of aac(6′)-Ib-cr-encoding plasmids increased the MIC of ciprofloxacin by 2-fold. In five of six KPC-P isolates, aac(6′)-Ib-cr and bla_KPC-2 were encoded on the same plasmid. Conclusions: The most prevalent PMQR gene in the studied KPC-PK. pneumoniae isolates is aac(6′)-Ib-cr. The co-existence of PMQR genes with KPC on the same plasmid poses a serious epidemiological, clinical and public-health threat.

Original language	English
Pages (from-to)	718-722
Number of pages	5
Journal	Journal of Antimicrobial Chemotherapy
Volume	64
Issue number	4
DOIs	https://doi.org/10.1093/jac/dkp272
State	Published - 2009
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Research Fund of Chief Scientist Office, Ministry of Health (Jerusalem, Israel), and by the Morasha 1833/07 grant from the Israel Science Foundation awarded to Dr Jacob Strahilevitz.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Carbapenem resistance
Plasmids
Quinolone resistance
β-lactamases

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10.1093/jac/dkp272

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title = "Detection of aac(6′)-Ib-cr in KPC-producing Klebsiella pneumoniae isolates from Tel Aviv, Israel",

abstract = "Objectives: We aimed to evaluate the occurrence and characteristics of plasmid-mediated quinolone resistance (PMQR) genes in KPC-producing (KPC-P) Klebsiella pneumoniae (Kpn) isolates in Tel Aviv Medical Center, Israel. Methods: Forty-seven KPC-P Kpn isolates were studied. Antibiotic susceptibilities were determined by Vitek 2, Etest or agar dilution. β-Lactamases and PMQR determinants were detected by PCR. For plasmid characterization, transformation, transconjugation, restriction mapping and Southern blot analysis were performed. Results: Six out of 47 (13\%) KPC-P isolates carried aac(6′)-Ib-cr. Acquisition of aac(6′)-Ib-cr-encoding plasmids increased the MIC of ciprofloxacin by 2-fold. In five of six KPC-P isolates, aac(6′)-Ib-cr and blaKPC-2 were encoded on the same plasmid. Conclusions: The most prevalent PMQR gene in the studied KPC-PK. pneumoniae isolates is aac(6′)-Ib-cr. The co-existence of PMQR genes with KPC on the same plasmid poses a serious epidemiological, clinical and public-health threat.",

keywords = "Carbapenem resistance, Plasmids, Quinolone resistance, β-lactamases",

author = "Inna Chmelnitsky and Orit Hermesh and Shiri Navon-Venezia and Jacob Strahilevitz and Yehuda Carmeli",

note = "Funding Information: This work was supported by the Research Fund of Chief Scientist Office, Ministry of Health (Jerusalem, Israel), and by the Morasha 1833/07 grant from the Israel Science Foundation awarded to Dr Jacob Strahilevitz.",

year = "2009",

doi = "10.1093/jac/dkp272",

language = "אנגלית",

volume = "64",

pages = "718--722",

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T1 - Detection of aac(6′)-Ib-cr in KPC-producing Klebsiella pneumoniae isolates from Tel Aviv, Israel

AU - Chmelnitsky, Inna

AU - Hermesh, Orit

AU - Navon-Venezia, Shiri

AU - Strahilevitz, Jacob

AU - Carmeli, Yehuda

N1 - Funding Information: This work was supported by the Research Fund of Chief Scientist Office, Ministry of Health (Jerusalem, Israel), and by the Morasha 1833/07 grant from the Israel Science Foundation awarded to Dr Jacob Strahilevitz.

PY - 2009

Y1 - 2009

N2 - Objectives: We aimed to evaluate the occurrence and characteristics of plasmid-mediated quinolone resistance (PMQR) genes in KPC-producing (KPC-P) Klebsiella pneumoniae (Kpn) isolates in Tel Aviv Medical Center, Israel. Methods: Forty-seven KPC-P Kpn isolates were studied. Antibiotic susceptibilities were determined by Vitek 2, Etest or agar dilution. β-Lactamases and PMQR determinants were detected by PCR. For plasmid characterization, transformation, transconjugation, restriction mapping and Southern blot analysis were performed. Results: Six out of 47 (13%) KPC-P isolates carried aac(6′)-Ib-cr. Acquisition of aac(6′)-Ib-cr-encoding plasmids increased the MIC of ciprofloxacin by 2-fold. In five of six KPC-P isolates, aac(6′)-Ib-cr and blaKPC-2 were encoded on the same plasmid. Conclusions: The most prevalent PMQR gene in the studied KPC-PK. pneumoniae isolates is aac(6′)-Ib-cr. The co-existence of PMQR genes with KPC on the same plasmid poses a serious epidemiological, clinical and public-health threat.

AB - Objectives: We aimed to evaluate the occurrence and characteristics of plasmid-mediated quinolone resistance (PMQR) genes in KPC-producing (KPC-P) Klebsiella pneumoniae (Kpn) isolates in Tel Aviv Medical Center, Israel. Methods: Forty-seven KPC-P Kpn isolates were studied. Antibiotic susceptibilities were determined by Vitek 2, Etest or agar dilution. β-Lactamases and PMQR determinants were detected by PCR. For plasmid characterization, transformation, transconjugation, restriction mapping and Southern blot analysis were performed. Results: Six out of 47 (13%) KPC-P isolates carried aac(6′)-Ib-cr. Acquisition of aac(6′)-Ib-cr-encoding plasmids increased the MIC of ciprofloxacin by 2-fold. In five of six KPC-P isolates, aac(6′)-Ib-cr and blaKPC-2 were encoded on the same plasmid. Conclusions: The most prevalent PMQR gene in the studied KPC-PK. pneumoniae isolates is aac(6′)-Ib-cr. The co-existence of PMQR genes with KPC on the same plasmid poses a serious epidemiological, clinical and public-health threat.

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KW - Plasmids

KW - Quinolone resistance

KW - β-lactamases

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Detection of aac(6′)-Ib-cr in KPC-producing Klebsiella pneumoniae isolates from Tel Aviv, Israel

Abstract

Bibliographical note

UN SDGs

Keywords

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