Abstract
Solid-state nanopores can be used as affordable and user-friendly detectors for biological molecules such as DNA, RNA, and proteins. Here, we used chemically modified silicon nitride nanopores to detect nanoparticles. The nanopores were modified with a peptide-based coating that significantly improved the pore current stability when compared to uncoated pores. The translocation of charged gold nanoparticles through the nanopore was characterized both for single particles (monomers) and for structures of two particles (dimers), connected by individual double-stranded DNA molecules. The monomers and dimers differ in their translocation time through the nanopore. The developed nanopore/nanoparticle-based system is a promising platform for the detection of DNA and other molecules for diagnostic purposes.
Original language | American English |
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Pages (from-to) | 1000-1008 |
Number of pages | 9 |
Journal | ACS Applied Nano Materials |
Volume | 4 |
Issue number | 2 |
DOIs | |
State | Published - 26 Feb 2021 |
Bibliographical note
Funding Information:This work was supported by the Israel Science Foundation (ISF grant 1589/14), the ISF–NSFC (grant 2556/17), the Ministry of Science & Technology, Israel (grant 3-16840), and the Minerva Centre for bio-hybrid complex systems. D.P. thanks Etta and Paul Schankerman Chair of Molecular Biomedicine.
Publisher Copyright:
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Keywords
- biomarkers
- dimers
- gold nanoparticles
- nanopores
- peptides