Detection of Au Nanoparticles Using Peptide-Modified Si3N4Nanopores

Abeer Karmi; Haya Dachlika; Gowri Priya Sakala; Dvir Rotem; Meital Reches; Danny Porath

doi:10.1021/acsanm.0c02126

Detection of Au Nanoparticles Using Peptide-Modified Si₃N₄Nanopores

Abeer Karmi, Haya Dachlika, Gowri Priya Sakala, Dvir Rotem, Meital Reches^*, Danny Porath^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Solid-state nanopores can be used as affordable and user-friendly detectors for biological molecules such as DNA, RNA, and proteins. Here, we used chemically modified silicon nitride nanopores to detect nanoparticles. The nanopores were modified with a peptide-based coating that significantly improved the pore current stability when compared to uncoated pores. The translocation of charged gold nanoparticles through the nanopore was characterized both for single particles (monomers) and for structures of two particles (dimers), connected by individual double-stranded DNA molecules. The monomers and dimers differ in their translocation time through the nanopore. The developed nanopore/nanoparticle-based system is a promising platform for the detection of DNA and other molecules for diagnostic purposes.

Original language	American English
Pages (from-to)	1000-1008
Number of pages	9
Journal	ACS Applied Nano Materials
Volume	4
Issue number	2
DOIs	https://doi.org/10.1021/acsanm.0c02126
State	Published - 26 Feb 2021

Bibliographical note

Funding Information:
This work was supported by the Israel Science Foundation (ISF grant 1589/14), the ISF–NSFC (grant 2556/17), the Ministry of Science & Technology, Israel (grant 3-16840), and the Minerva Centre for bio-hybrid complex systems. D.P. thanks Etta and Paul Schankerman Chair of Molecular Biomedicine.

Publisher Copyright:
©

Keywords

biomarkers
dimers
gold nanoparticles
nanopores
peptides

Access to Document

10.1021/acsanm.0c02126

Cite this

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abstract = "Solid-state nanopores can be used as affordable and user-friendly detectors for biological molecules such as DNA, RNA, and proteins. Here, we used chemically modified silicon nitride nanopores to detect nanoparticles. The nanopores were modified with a peptide-based coating that significantly improved the pore current stability when compared to uncoated pores. The translocation of charged gold nanoparticles through the nanopore was characterized both for single particles (monomers) and for structures of two particles (dimers), connected by individual double-stranded DNA molecules. The monomers and dimers differ in their translocation time through the nanopore. The developed nanopore/nanoparticle-based system is a promising platform for the detection of DNA and other molecules for diagnostic purposes.",

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AU - Sakala, Gowri Priya

AU - Rotem, Dvir

AU - Reches, Meital

AU - Porath, Danny

N1 - Funding Information: This work was supported by the Israel Science Foundation (ISF grant 1589/14), the ISF–NSFC (grant 2556/17), the Ministry of Science & Technology, Israel (grant 3-16840), and the Minerva Centre for bio-hybrid complex systems. D.P. thanks Etta and Paul Schankerman Chair of Molecular Biomedicine. Publisher Copyright: ©

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