Detection of Bartonella spp. in wild rodents in Israel using HRM real-time PCR

Danny Morick; Gad Baneth; Boaz Avidor; Michael Y. Kosoy; Kosta Y. Mumcuoglu; Dvir Mintz; Osnat Eyal; Ralph Goethe; Andreas Mietze; Nahum Shpigel; Shimon Harrus

doi:10.1016/j.vetmic.2009.06.019

Detection of Bartonella spp. in wild rodents in Israel using HRM real-time PCR

Danny Morick, Gad Baneth, Boaz Avidor, Michael Y. Kosoy, Kosta Y. Mumcuoglu, Dvir Mintz, Osnat Eyal, Ralph Goethe, Andreas Mietze, Nahum Shpigel, Shimon Harrus^*

^*Corresponding author for this work

The Koret School of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

The prevalence of Bartonella spp. in wild rodents was studied in 19 geographical locations in Israel. One hundred and twelve rodents belonging to five species (Mus musculus, Rattus rattus, Microtus socialis, Acomys cahirinus and Apodemus sylvaticus) were included in the survey. In addition, 156 ectoparasites were collected from the rodents. Spleen sample from each rodent and the ectoparasites were examined for the presence of Bartonella DNA using high resolution melt (HRM) real-time PCR. The method was designed for the simultaneous detection and differentiation of eight Bartonella spp. according to the nucleotide variation in each of two gene fragments (rpoB and gltA) and the 16S-23S intergenic spacer (ITS) locus, using the same PCR protocol which allowed the simultaneous amplification of the three different loci. Bartonella DNA was detected in spleen samples of 19 out of 79 (24%) black rats (R. rattus) and in 1 of 4 (25%) Cairo spiny mice (A. cahirinus). In addition, 15 of 34 (44%) flea pools harbored Bartonella DNA. Only rat flea (Xenopsyla cheopis) pools collected from black rats (R. rattus) were positive for Bartonella DNA. The Bartonella sp. detected in spleen samples from black rats (R. rattus) was closely related to both B. tribocorum and B. elizabethae. The species detected in the Cairo spiny mouse (A. cahirinus) spleen sample was closely related to the zoonotic pathogen, B. elizabethae. These results indicate that Bartonella species are highly prevalent in suburban rodent populations and their ectoparasites in Israel. Further investigation of the prevalence and zoonotic potential of the Bartonella species detected in the black rats and the Cairo spiny mouse is warranted.

Original language	English
Pages (from-to)	293-297
Number of pages	5
Journal	Veterinary Microbiology
Volume	139
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetmic.2009.06.019
State	Published - 18 Nov 2009

Bibliographical note

Funding Information:
This study was sponsored by The Niedersachsen-Israel cooperation fund (# VWZN2176). The authors would like to acknowledge Dr. Günter C. Müller for his professional assistance.

Keywords

Acomys cahirinus
Bartonella elizabethae
Bartonella tribocorum
HRM real-time PCR
Rattus rattus
Zoonosis

Access to Document

10.1016/j.vetmic.2009.06.019

Cite this

@article{53d464cdf171437bb0ecf2f067fe69b6,

title = "Detection of Bartonella spp. in wild rodents in Israel using HRM real-time PCR",

abstract = "The prevalence of Bartonella spp. in wild rodents was studied in 19 geographical locations in Israel. One hundred and twelve rodents belonging to five species (Mus musculus, Rattus rattus, Microtus socialis, Acomys cahirinus and Apodemus sylvaticus) were included in the survey. In addition, 156 ectoparasites were collected from the rodents. Spleen sample from each rodent and the ectoparasites were examined for the presence of Bartonella DNA using high resolution melt (HRM) real-time PCR. The method was designed for the simultaneous detection and differentiation of eight Bartonella spp. according to the nucleotide variation in each of two gene fragments (rpoB and gltA) and the 16S-23S intergenic spacer (ITS) locus, using the same PCR protocol which allowed the simultaneous amplification of the three different loci. Bartonella DNA was detected in spleen samples of 19 out of 79 (24%) black rats (R. rattus) and in 1 of 4 (25%) Cairo spiny mice (A. cahirinus). In addition, 15 of 34 (44%) flea pools harbored Bartonella DNA. Only rat flea (Xenopsyla cheopis) pools collected from black rats (R. rattus) were positive for Bartonella DNA. The Bartonella sp. detected in spleen samples from black rats (R. rattus) was closely related to both B. tribocorum and B. elizabethae. The species detected in the Cairo spiny mouse (A. cahirinus) spleen sample was closely related to the zoonotic pathogen, B. elizabethae. These results indicate that Bartonella species are highly prevalent in suburban rodent populations and their ectoparasites in Israel. Further investigation of the prevalence and zoonotic potential of the Bartonella species detected in the black rats and the Cairo spiny mouse is warranted.",

keywords = "Acomys cahirinus, Bartonella elizabethae, Bartonella tribocorum, HRM real-time PCR, Rattus rattus, Zoonosis",

author = "Danny Morick and Gad Baneth and Boaz Avidor and Kosoy, {Michael Y.} and Mumcuoglu, {Kosta Y.} and Dvir Mintz and Osnat Eyal and Ralph Goethe and Andreas Mietze and Nahum Shpigel and Shimon Harrus",

note = "Funding Information: This study was sponsored by The Niedersachsen-Israel cooperation fund (# VWZN2176). The authors would like to acknowledge Dr. G{\"u}nter C. M{\"u}ller for his professional assistance.",

year = "2009",

month = nov,

day = "18",

doi = "10.1016/j.vetmic.2009.06.019",

language = "אנגלית",

volume = "139",

pages = "293--297",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier B.V.",

number = "3-4",

}

TY - JOUR

T1 - Detection of Bartonella spp. in wild rodents in Israel using HRM real-time PCR

AU - Morick, Danny

AU - Baneth, Gad

AU - Avidor, Boaz

AU - Kosoy, Michael Y.

AU - Mumcuoglu, Kosta Y.

AU - Mintz, Dvir

AU - Eyal, Osnat

AU - Goethe, Ralph

AU - Mietze, Andreas

AU - Shpigel, Nahum

AU - Harrus, Shimon

N1 - Funding Information: This study was sponsored by The Niedersachsen-Israel cooperation fund (# VWZN2176). The authors would like to acknowledge Dr. Günter C. Müller for his professional assistance.

PY - 2009/11/18

Y1 - 2009/11/18

N2 - The prevalence of Bartonella spp. in wild rodents was studied in 19 geographical locations in Israel. One hundred and twelve rodents belonging to five species (Mus musculus, Rattus rattus, Microtus socialis, Acomys cahirinus and Apodemus sylvaticus) were included in the survey. In addition, 156 ectoparasites were collected from the rodents. Spleen sample from each rodent and the ectoparasites were examined for the presence of Bartonella DNA using high resolution melt (HRM) real-time PCR. The method was designed for the simultaneous detection and differentiation of eight Bartonella spp. according to the nucleotide variation in each of two gene fragments (rpoB and gltA) and the 16S-23S intergenic spacer (ITS) locus, using the same PCR protocol which allowed the simultaneous amplification of the three different loci. Bartonella DNA was detected in spleen samples of 19 out of 79 (24%) black rats (R. rattus) and in 1 of 4 (25%) Cairo spiny mice (A. cahirinus). In addition, 15 of 34 (44%) flea pools harbored Bartonella DNA. Only rat flea (Xenopsyla cheopis) pools collected from black rats (R. rattus) were positive for Bartonella DNA. The Bartonella sp. detected in spleen samples from black rats (R. rattus) was closely related to both B. tribocorum and B. elizabethae. The species detected in the Cairo spiny mouse (A. cahirinus) spleen sample was closely related to the zoonotic pathogen, B. elizabethae. These results indicate that Bartonella species are highly prevalent in suburban rodent populations and their ectoparasites in Israel. Further investigation of the prevalence and zoonotic potential of the Bartonella species detected in the black rats and the Cairo spiny mouse is warranted.

AB - The prevalence of Bartonella spp. in wild rodents was studied in 19 geographical locations in Israel. One hundred and twelve rodents belonging to five species (Mus musculus, Rattus rattus, Microtus socialis, Acomys cahirinus and Apodemus sylvaticus) were included in the survey. In addition, 156 ectoparasites were collected from the rodents. Spleen sample from each rodent and the ectoparasites were examined for the presence of Bartonella DNA using high resolution melt (HRM) real-time PCR. The method was designed for the simultaneous detection and differentiation of eight Bartonella spp. according to the nucleotide variation in each of two gene fragments (rpoB and gltA) and the 16S-23S intergenic spacer (ITS) locus, using the same PCR protocol which allowed the simultaneous amplification of the three different loci. Bartonella DNA was detected in spleen samples of 19 out of 79 (24%) black rats (R. rattus) and in 1 of 4 (25%) Cairo spiny mice (A. cahirinus). In addition, 15 of 34 (44%) flea pools harbored Bartonella DNA. Only rat flea (Xenopsyla cheopis) pools collected from black rats (R. rattus) were positive for Bartonella DNA. The Bartonella sp. detected in spleen samples from black rats (R. rattus) was closely related to both B. tribocorum and B. elizabethae. The species detected in the Cairo spiny mouse (A. cahirinus) spleen sample was closely related to the zoonotic pathogen, B. elizabethae. These results indicate that Bartonella species are highly prevalent in suburban rodent populations and their ectoparasites in Israel. Further investigation of the prevalence and zoonotic potential of the Bartonella species detected in the black rats and the Cairo spiny mouse is warranted.

KW - Acomys cahirinus

KW - Bartonella elizabethae

KW - Bartonella tribocorum

KW - HRM real-time PCR

KW - Rattus rattus

KW - Zoonosis

UR - http://www.scopus.com/inward/record.url?scp=70349913648&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2009.06.019

DO - 10.1016/j.vetmic.2009.06.019

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 19595521

AN - SCOPUS:70349913648

SN - 0378-1135

VL - 139

SP - 293

EP - 297

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 3-4

ER -

Detection of Bartonella spp. in wild rodents in Israel using HRM real-time PCR

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this