TY - JOUR
T1 - Detection of bird schistosomes in lakes by PCR and filter-hybridization
AU - Hertel, Jan
AU - Hamburger, Joseph
AU - Haberl, Bernhard
AU - Haas, Wilfried
PY - 2002/5/1
Y1 - 2002/5/1
N2 - Many lakes around the world are contaminated with bird schistosome cercariae, which penetrate into human skin, causing an itching dermatitis called "swimmers' itch." Bathers could be forewarned from exposure to the larvae and ecological examinations could be performed, when a sensitive method to detect the parasites in aquatic systems, where lots of organisms hinder microscopic examinations, would be available. For this purpose we cloned, sequenced, and analyzed a 396bp tandem repeated DNA sequence from Trichobilharzia ocellata (ToSau3A), and employed it for developing molecular detection assays. It hybridized with less than 100pg DNA from different Trichobilharzia species (T. ocellata, Trichobilharzia franki, and Trichobilharzia regenti), but not with 10ng DNA from other related or sympatric trematodes. A PCR assay, amplifying this sequence with the same specificity, detected 100fg T. ocellata DNA, 1 cercaria in 0.5g plankton, and 2 cercariae in 0.5g host snail tissues.
AB - Many lakes around the world are contaminated with bird schistosome cercariae, which penetrate into human skin, causing an itching dermatitis called "swimmers' itch." Bathers could be forewarned from exposure to the larvae and ecological examinations could be performed, when a sensitive method to detect the parasites in aquatic systems, where lots of organisms hinder microscopic examinations, would be available. For this purpose we cloned, sequenced, and analyzed a 396bp tandem repeated DNA sequence from Trichobilharzia ocellata (ToSau3A), and employed it for developing molecular detection assays. It hybridized with less than 100pg DNA from different Trichobilharzia species (T. ocellata, Trichobilharzia franki, and Trichobilharzia regenti), but not with 10ng DNA from other related or sympatric trematodes. A PCR assay, amplifying this sequence with the same specificity, detected 100fg T. ocellata DNA, 1 cercaria in 0.5g plankton, and 2 cercariae in 0.5g host snail tissues.
KW - Detection
KW - Monitoring
KW - Repeated DNA
KW - Trematode
KW - Trichobilharzia
UR - http://www.scopus.com/inward/record.url?scp=0036591302&partnerID=8YFLogxK
U2 - 10.1016/S0014-4894(02)00036-X
DO - 10.1016/S0014-4894(02)00036-X
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C2 - 12243738
AN - SCOPUS:0036591302
SN - 0014-4894
VL - 101
SP - 57
EP - 63
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 1
ER -