TY - JOUR
T1 - Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids
AU - Vollmer, Amy C.
AU - Belkin, Shimshon
AU - Smulski, Dana R.
AU - Van Dyk, Tina K.
AU - Larossa, Robert A.
PY - 1997/7
Y1 - 1997/7
N2 - Plasmids were constructed in which DNA damage-inducible promoters recA, uvrA, and alkA from Escherichia coli were fused to the Vibrio fischeri luxCDABE operon. Introduction of these plasmids into E. coli allowed the detection of a dose-dependent response to DNA-damaging agents, such as mitomycin and UV irradiation. Bioluminescence was measured in real time over extended periods. The fusion of the recA promoter to luxCDABE showed the most dramatic and sensitive responses. lexA dependence of the bioluminescent SOS response was demonstrated, confirming that this biosensor's reports were transmitted by the expected regulatory circuitry. Comparisons were made between luxCDABE and lacZ fusions to each promoter. It is suggested that the lux biosensors may have use in monitoring chemical, physical, and genotoxic agents as well as in further characterizing the mechanisms of DNA repair.
AB - Plasmids were constructed in which DNA damage-inducible promoters recA, uvrA, and alkA from Escherichia coli were fused to the Vibrio fischeri luxCDABE operon. Introduction of these plasmids into E. coli allowed the detection of a dose-dependent response to DNA-damaging agents, such as mitomycin and UV irradiation. Bioluminescence was measured in real time over extended periods. The fusion of the recA promoter to luxCDABE showed the most dramatic and sensitive responses. lexA dependence of the bioluminescent SOS response was demonstrated, confirming that this biosensor's reports were transmitted by the expected regulatory circuitry. Comparisons were made between luxCDABE and lacZ fusions to each promoter. It is suggested that the lux biosensors may have use in monitoring chemical, physical, and genotoxic agents as well as in further characterizing the mechanisms of DNA repair.
UR - http://www.scopus.com/inward/record.url?scp=0030751305&partnerID=8YFLogxK
U2 - 10.1128/aem.63.7.2566-2571.1997
DO - 10.1128/aem.63.7.2566-2571.1997
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C2 - 9212407
AN - SCOPUS:0030751305
SN - 0099-2240
VL - 63
SP - 2566
EP - 2571
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 7
ER -